p47phox is a cytosolic component of the phagocyte NADPH oxidase, which is responsible for the production of the superoxide which kills invasive microorgamisms. A recombinant form of a histidine-tagged tandem SH3 domain of the p47phox-containing polybasic autoinhibited region was expressed in Escherichia coli and purified and crystallized by the sitting-drop vapour-diffusion method at 293 K using polyethylene glycol 6000 as a precipitant. Diffraction data were collected to 2.15 Å resolution at 100 K using synchrotron radiation. The crystal belongs to space group P4 1212 or P43212, with unit-cell parameters a = 100.02, c = 44.94 Å. The presence of one molecule per asymmetric unit gives a crystal volume per protein mass (VM) of 2.6 Å3 Da-1 and a solvent content of 52% by volume.
|Number of pages||2|
|Journal||Acta Crystallographica - Section D Biological Crystallography|
|Publication status||Published - Aug 1 2003|
All Science Journal Classification (ASJC) codes
- Structural Biology