TY - JOUR
T1 - Crystallization and preliminary X-ray analysis of the mRNA-binding domain of elongation factor SelB in complex with RNA
AU - Rasubala, Linda
AU - Fourmy, Dominique
AU - Ose, Toyoyuki
AU - Kohda, Daisuke
AU - Maenaka, Katsumi
AU - Yoshizawa, Satoko
PY - 2005
Y1 - 2005
N2 - In bacteria, the selenocysteine-specific elongation factor SelB is necessary for incorporation of selenocysteine, the 21st amino acid, into proteins by the ribosome. SelB binds to an mRNA hairpin formed by the selenocysteine-insertion sequence (SECIS) and delivers selenocysteyl-tRNA (Sec-tRNASec) at the ribosomal A site. The minimum fragment (residues 512-634) of Moorella thermoacetica SelB (SelB-M) required for mRNA binding has been overexpressed and purified. The complex of SelB-M with 23 nucleotides of the SECIS mRNA hairpin was crystallized at 293 K using the hanging-drop vapour-diffusion or oil-batch methods. The crystals diffract to 2.3 Å resolution using SPring-8 BL41XU and belong to the space group P2 1212, with unit-cell parameters a = 81.69, b = 169.58, c = 71.69 Å.
AB - In bacteria, the selenocysteine-specific elongation factor SelB is necessary for incorporation of selenocysteine, the 21st amino acid, into proteins by the ribosome. SelB binds to an mRNA hairpin formed by the selenocysteine-insertion sequence (SECIS) and delivers selenocysteyl-tRNA (Sec-tRNASec) at the ribosomal A site. The minimum fragment (residues 512-634) of Moorella thermoacetica SelB (SelB-M) required for mRNA binding has been overexpressed and purified. The complex of SelB-M with 23 nucleotides of the SECIS mRNA hairpin was crystallized at 293 K using the hanging-drop vapour-diffusion or oil-batch methods. The crystals diffract to 2.3 Å resolution using SPring-8 BL41XU and belong to the space group P2 1212, with unit-cell parameters a = 81.69, b = 169.58, c = 71.69 Å.
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U2 - 10.1107/S1744309105003611
DO - 10.1107/S1744309105003611
M3 - Article
C2 - 16511023
AN - SCOPUS:33646488074
SN - 1744-3091
VL - 61
SP - 296
EP - 298
JO - Acta Crystallographica Section F:Structural Biology Communications
JF - Acta Crystallographica Section F:Structural Biology Communications
IS - 3
ER -