Crystallization and preliminary X-ray analysis of the tRNA thiolation enzyme MnmA from Escherichia coli complexed with tRNAGlu

Tomoyuki Numata; Yoshiho Ikeuchi; Shuya Fukai; Hiroaki Adachi; Hiroyoshi Matsumura; Kazufumi Takano; Satoshi Murakami; Tsuyoshi Inoue; Yusuke Mori; Takatomo Sasaki; Tsutomu Suzuki; Osamu Nureki

doi:10.1107/S174430910600738X

Crystallization and preliminary X-ray analysis of the tRNA thiolation enzyme MnmA from Escherichia coli complexed with tRNA^Glu

Tomoyuki Numata, Yoshiho Ikeuchi, Shuya Fukai, Hiroaki Adachi, Hiroyoshi Matsumura, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Takatomo Sasaki, Tsutomu Suzuki, Osamu Nureki

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

MnmA catalyzes a sulfuration reaction to synthesize 2-thiouridine at the wobble positions of tRNA^Glu, tRNA^Gln and tRNA ^Lys in Escherichia coli. The binary complex of MnmA and tRNA ^Glu was crystallized in two different crystal forms: forms I and II. Cocrystallization of MnmA-tRNA^Glu with ATP yielded form III crystals. The three crystal forms diffracted to 3.1, 3.4 and 3.4 Å resolution, respectively, using synchrotron radiation at SPring-8. These crystals belong to space groups C2, I2₁2₁2₁ and C2, with unit-cell parameters a = 225.4, b = 175.8, c = 53.0 Å, β = 101.6°, a = 101.5, b = 108.0, c = 211.2 Å and a = 238.1, b = 102.1, c = 108.2 Å, β = 117.0°, respectively. The asymmetric units of these crystals are expected to contain two, one and two MnmA-tRNA^Glu complexes, respectively.

Original language	English
Pages (from-to)	368-371
Number of pages	4
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	62
Issue number	4
DOIs	https://doi.org/10.1107/S174430910600738X
Publication status	Published - Apr 2006
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

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10.1107/S174430910600738X

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Numata, T., Ikeuchi, Y., Fukai, S., Adachi, H., Matsumura, H., Takano, K., Murakami, S., Inoue, T., Mori, Y., Sasaki, T., Suzuki, T., & Nureki, O. (2006). Crystallization and preliminary X-ray analysis of the tRNA thiolation enzyme MnmA from Escherichia coli complexed with tRNA^Glu. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 62(4), 368-371. https://doi.org/10.1107/S174430910600738X

Crystallization and preliminary X-ray analysis of the tRNA thiolation enzyme MnmA from Escherichia coli complexed with tRNA^Glu. / Numata, Tomoyuki; Ikeuchi, Yoshiho; Fukai, Shuya et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 62, No. 4, 04.2006, p. 368-371.

Research output: Contribution to journal › Article › peer-review

Numata, T, Ikeuchi, Y, Fukai, S, Adachi, H, Matsumura, H, Takano, K, Murakami, S, Inoue, T, Mori, Y, Sasaki, T, Suzuki, T & Nureki, O 2006, 'Crystallization and preliminary X-ray analysis of the tRNA thiolation enzyme MnmA from Escherichia coli complexed with tRNA^Glu', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 62, no. 4, pp. 368-371. https://doi.org/10.1107/S174430910600738X

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title = "Crystallization and preliminary X-ray analysis of the tRNA thiolation enzyme MnmA from Escherichia coli complexed with tRNAGlu",

abstract = "MnmA catalyzes a sulfuration reaction to synthesize 2-thiouridine at the wobble positions of tRNAGlu, tRNAGln and tRNA Lys in Escherichia coli. The binary complex of MnmA and tRNA Glu was crystallized in two different crystal forms: forms I and II. Cocrystallization of MnmA-tRNAGlu with ATP yielded form III crystals. The three crystal forms diffracted to 3.1, 3.4 and 3.4 {\AA} resolution, respectively, using synchrotron radiation at SPring-8. These crystals belong to space groups C2, I212121 and C2, with unit-cell parameters a = 225.4, b = 175.8, c = 53.0 {\AA}, β = 101.6°, a = 101.5, b = 108.0, c = 211.2 {\AA} and a = 238.1, b = 102.1, c = 108.2 {\AA}, β = 117.0°, respectively. The asymmetric units of these crystals are expected to contain two, one and two MnmA-tRNAGlu complexes, respectively.",

author = "Tomoyuki Numata and Yoshiho Ikeuchi and Shuya Fukai and Hiroaki Adachi and Hiroyoshi Matsumura and Kazufumi Takano and Satoshi Murakami and Tsuyoshi Inoue and Yusuke Mori and Takatomo Sasaki and Tsutomu Suzuki and Osamu Nureki",

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doi = "10.1107/S174430910600738X",

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T1 - Crystallization and preliminary X-ray analysis of the tRNA thiolation enzyme MnmA from Escherichia coli complexed with tRNAGlu

AU - Numata, Tomoyuki

AU - Ikeuchi, Yoshiho

AU - Fukai, Shuya

AU - Adachi, Hiroaki

AU - Matsumura, Hiroyoshi

AU - Takano, Kazufumi

AU - Murakami, Satoshi

AU - Inoue, Tsuyoshi

AU - Mori, Yusuke

AU - Sasaki, Takatomo

AU - Suzuki, Tsutomu

AU - Nureki, Osamu

PY - 2006/4

Y1 - 2006/4

N2 - MnmA catalyzes a sulfuration reaction to synthesize 2-thiouridine at the wobble positions of tRNAGlu, tRNAGln and tRNA Lys in Escherichia coli. The binary complex of MnmA and tRNA Glu was crystallized in two different crystal forms: forms I and II. Cocrystallization of MnmA-tRNAGlu with ATP yielded form III crystals. The three crystal forms diffracted to 3.1, 3.4 and 3.4 Å resolution, respectively, using synchrotron radiation at SPring-8. These crystals belong to space groups C2, I212121 and C2, with unit-cell parameters a = 225.4, b = 175.8, c = 53.0 Å, β = 101.6°, a = 101.5, b = 108.0, c = 211.2 Å and a = 238.1, b = 102.1, c = 108.2 Å, β = 117.0°, respectively. The asymmetric units of these crystals are expected to contain two, one and two MnmA-tRNAGlu complexes, respectively.

AB - MnmA catalyzes a sulfuration reaction to synthesize 2-thiouridine at the wobble positions of tRNAGlu, tRNAGln and tRNA Lys in Escherichia coli. The binary complex of MnmA and tRNA Glu was crystallized in two different crystal forms: forms I and II. Cocrystallization of MnmA-tRNAGlu with ATP yielded form III crystals. The three crystal forms diffracted to 3.1, 3.4 and 3.4 Å resolution, respectively, using synchrotron radiation at SPring-8. These crystals belong to space groups C2, I212121 and C2, with unit-cell parameters a = 225.4, b = 175.8, c = 53.0 Å, β = 101.6°, a = 101.5, b = 108.0, c = 211.2 Å and a = 238.1, b = 102.1, c = 108.2 Å, β = 117.0°, respectively. The asymmetric units of these crystals are expected to contain two, one and two MnmA-tRNAGlu complexes, respectively.

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Crystallization and preliminary X-ray analysis of the tRNA thiolation enzyme MnmA from Escherichia coli complexed with tRNA^Glu

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