Crystallization and preliminary X-ray crystallographic study of [NiFe]-hydrogenase maturation factor HypE from Thermococcus kodakaraensis KOD1

Takayuki Arai, Satoshi Watanabe, Rie Matsumi, Haruyuki Atomi, Tadayuki Imanaka, Kunio Miki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The hydrogenase maturation protein HypE is involved in the biosynthesis of the CN ligands of the active-site iron of [NiFe] hydrogenases using carbamoylphosphate as a substrate. Here, the crystallization and preliminary crystallographic analysis of HypE from Thermococcus kodakaraensis KOD1 are reported. Crystals of HypE (338 amino acids, 35.9 kDa) have been obtained by the sitting-drop vapour-diffusion method using 2-methyl-2,4-pentanediol (MPD) as a precipitant. The crystals belong to space group P21212, with unit-cell parameters a = 88.3, b = 45.8, c = 75.1 Å. There is one HypE molecule in the asymmetric unit. A complete native X-ray diffraction data set was collected to a maximum resolution of 1.55 Å at 100 K.

Original languageEnglish
Pages (from-to)765-767
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume63
Issue number9
DOIs
Publication statusPublished - Aug 10 2007

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Thermococcus
Hydrogenase
Crystallization
X-Ray Diffraction
Catalytic Domain
X-Rays
crystallization
Ligands
Amino Acids
X rays
Crystals
biosynthesis
Biosynthesis
crystals
amino acids
Proteins
x rays
Iron
Vapors
vapors

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

Crystallization and preliminary X-ray crystallographic study of [NiFe]-hydrogenase maturation factor HypE from Thermococcus kodakaraensis KOD1. / Arai, Takayuki; Watanabe, Satoshi; Matsumi, Rie; Atomi, Haruyuki; Imanaka, Tadayuki; Miki, Kunio.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 63, No. 9, 10.08.2007, p. 765-767.

Research output: Contribution to journalArticle

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