TY - JOUR
T1 - Crystallization and preliminary X-ray crystallographic study of the ribosomal protein S7 from Bacillus stearothermophilus
AU - Harada, Nao
AU - Sano, Kazunari
AU - Kimura, Makoto
AU - Hosaka, Harumi
AU - Nakagawa, Atushi
AU - Tanaka, Isao
N1 - Funding Information:
The Kyushu group thanks Professor N. Yamasaki (Kyushu University) for his encouragement and interest in the research. This work was supported in part by a grant-in-aid from the Ministry of Education, Science and Culture of Japan. A.N. and I.T. are members of the TARA project of the University of Tsukuba, Japan. We thank Professor N. Sakabe, Dr. N. Watanabe, and Dr. M. Suzuki of the Photon Factory for their kind help in data collection.
PY - 1997/10
Y1 - 1997/10
N2 - Overproduction and crystallization of Bacillus stearothermophilus ribosomal protein S7 (BstS7), a primary 16S rRNA binding protein and also a translational repressor protein, have been performed to analyze its three- dimensional structure by X-ray crystallography. Ribosomal protein BstS7 was expressed in the cytoplasmic fraction of the E. coli cells and purified to homogeneity. This recombinant BstS7 was used to produce crystals with P21 symmetry that diffracted to 2.5 Å resolution which are suitable for high- resolution X-ray crystallographic analysis.
AB - Overproduction and crystallization of Bacillus stearothermophilus ribosomal protein S7 (BstS7), a primary 16S rRNA binding protein and also a translational repressor protein, have been performed to analyze its three- dimensional structure by X-ray crystallography. Ribosomal protein BstS7 was expressed in the cytoplasmic fraction of the E. coli cells and purified to homogeneity. This recombinant BstS7 was used to produce crystals with P21 symmetry that diffracted to 2.5 Å resolution which are suitable for high- resolution X-ray crystallographic analysis.
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U2 - 10.1006/jsbi.1997.3907
DO - 10.1006/jsbi.1997.3907
M3 - Article
C2 - 9356300
AN - SCOPUS:0031257472
VL - 120
SP - 112
EP - 114
JO - Journal of Structural Biology
JF - Journal of Structural Biology
SN - 1047-8477
IS - 1
ER -