Crystallization and preliminary X-ray crystallographic study of the ribosomal protein S7 from Bacillus stearothermophilus

Nao Harada, Kazunari Sano, Makoto Kimura, Harumi Hosaka, Atushi Nakagawa, Isao Tanaka

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Overproduction and crystallization of Bacillus stearothermophilus ribosomal protein S7 (BstS7), a primary 16S rRNA binding protein and also a translational repressor protein, have been performed to analyze its three- dimensional structure by X-ray crystallography. Ribosomal protein BstS7 was expressed in the cytoplasmic fraction of the E. coli cells and purified to homogeneity. This recombinant BstS7 was used to produce crystals with P21 symmetry that diffracted to 2.5 Å resolution which are suitable for high- resolution X-ray crystallographic analysis.

Original languageEnglish
Pages (from-to)112-114
Number of pages3
JournalJournal of structural biology
Volume120
Issue number1
DOIs
Publication statusPublished - Jan 1 1997

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Geobacillus stearothermophilus
Crystallization
X-Rays
Repressor Proteins
Ribosomal Proteins
X Ray Crystallography
Carrier Proteins
Escherichia coli
ribosomal protein S7

All Science Journal Classification (ASJC) codes

  • Structural Biology

Cite this

Crystallization and preliminary X-ray crystallographic study of the ribosomal protein S7 from Bacillus stearothermophilus. / Harada, Nao; Sano, Kazunari; Kimura, Makoto; Hosaka, Harumi; Nakagawa, Atushi; Tanaka, Isao.

In: Journal of structural biology, Vol. 120, No. 1, 01.01.1997, p. 112-114.

Research output: Contribution to journalArticle

Harada, Nao ; Sano, Kazunari ; Kimura, Makoto ; Hosaka, Harumi ; Nakagawa, Atushi ; Tanaka, Isao. / Crystallization and preliminary X-ray crystallographic study of the ribosomal protein S7 from Bacillus stearothermophilus. In: Journal of structural biology. 1997 ; Vol. 120, No. 1. pp. 112-114.
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