Crystallization and preliminary X-ray diffraction analysis of a class v chitinase from Nicotiana tabacum

Takayuki Ohnuma, Takuo Osawa, Tamo Fukamizo, Tomoyuki Numata

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The plant chitinases, which have been implicated in self-defence against pathogens, are divided into at least five classes (classes I, II, III, IV and V). Although the crystal structures of several plant chitinases have been solved, no crystal structure of a class V chitinase has been reported to date. Here, the crystallization of Nicotiana tabacum class V chitinase (NtChiV) using the vapour-diffusion method is reported. The NtChiV crystals diffracted to 1.2 Å resolution using synchrotron radiation at the Photon Factory. The crystals belonged to the orthorhombic space group P21212, with unit-cell parameters a = 62.4, b = 120.3, c = 51.9 Å. The asymmetric unit of the crystals is expected to contain one molecule.

Original languageEnglish
Pages (from-to)1599-1601
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume66
Issue number12
DOIs
Publication statusPublished - Dec 1 2010
Externally publishedYes

Fingerprint

Chitinases
Crystallization
X-Ray Diffraction
X ray diffraction analysis
Tobacco
crystallization
diffraction
Crystals
x rays
Crystal structure
crystals
Plant Structures
Synchrotrons
crystal structure
pathogens
Pathogens
Synchrotron radiation
industrial plants
Photons
Industrial plants

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

Crystallization and preliminary X-ray diffraction analysis of a class v chitinase from Nicotiana tabacum. / Ohnuma, Takayuki; Osawa, Takuo; Fukamizo, Tamo; Numata, Tomoyuki.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 66, No. 12, 01.12.2010, p. 1599-1601.

Research output: Contribution to journalArticle

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