Crystallization and preliminary X-ray diffraction studies of 3-methyladenine-DNA glycosylase II from Escherichia coli

Yuriko Yamagata, Kyoko Odawara, Ken Ichi Tomita, Yusaku Nakabeppu, Mutsuo Sekiguchi

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Abstract

Single crystals of 3-methyladenine-DNA glycosylase II produced from the alkA gene of Escherichia coli have been obtained by the method of vapour diffusion with polyethylene glycol 6000 as precipitant at pH 8.5. The crystals belong to the monoclinic space group C2, with a = 58.6 A ̊, b = 76.8 A ̊, c = 62.2 A ̊, β = 110.3 °. They contain one molecule per asymmetric unit and diffract to 2.7 Å resolution.

Original languageEnglish
Pages (from-to)1055-1056
Number of pages2
JournalJournal of Molecular Biology
Volume204
Issue number4
DOIs
Publication statusPublished - Dec 20 1988

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All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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