TY - JOUR
T1 - Crystallization and preliminary X-ray studies of two serine proteinase inhibitors, BGIA and BGIT from the seeds of bitter gourd
AU - Kondo, Hidemasa
AU - Nakanose, Jin
AU - Nakagawa, Atsushi
AU - Tanaka, Isao
AU - Kimura, Makoto
AU - Funatsu, Gunki
PY - 1997/11
Y1 - 1997/11
N2 - Two serine proteinase inhibitors from seeds of the bitter gourd, BGIA (bitter gourd inhibitor against acidic amino acid-specific proteinase of Streptomyces griseus) and BGIT (bitter gourd trypsin inhibitor), were crystallized for X-ray structure determination. Crystals of BGIA belong to the monoclinic space group C2 with cell dimensions of a = 54.0 Å, b = 23.7 Å, c = 47.9 Å, and β = 105.4°, and diffracted X-ray up to 1.5 Å resolution. Crystals of BGIT belong to the triclinic space group P1 with cell dimensions of a = 22.8 Å, b = 23.5 Å, c = 28.4 Å, α = 93.1°, β = 99.6°, and γ = 101.0°, giving X-ray diffraction of over 1.2 Å resolution. Intensity data of BGIA and BGIT crystals were collected using synchrotron radiation up to 1.7 and 1.4 Å, respectively.
AB - Two serine proteinase inhibitors from seeds of the bitter gourd, BGIA (bitter gourd inhibitor against acidic amino acid-specific proteinase of Streptomyces griseus) and BGIT (bitter gourd trypsin inhibitor), were crystallized for X-ray structure determination. Crystals of BGIA belong to the monoclinic space group C2 with cell dimensions of a = 54.0 Å, b = 23.7 Å, c = 47.9 Å, and β = 105.4°, and diffracted X-ray up to 1.5 Å resolution. Crystals of BGIT belong to the triclinic space group P1 with cell dimensions of a = 22.8 Å, b = 23.5 Å, c = 28.4 Å, α = 93.1°, β = 99.6°, and γ = 101.0°, giving X-ray diffraction of over 1.2 Å resolution. Intensity data of BGIA and BGIT crystals were collected using synchrotron radiation up to 1.7 and 1.4 Å, respectively.
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U2 - 10.1006/jsbi.1997.3931
DO - 10.1006/jsbi.1997.3931
M3 - Article
C2 - 9417986
AN - SCOPUS:0031456307
VL - 120
SP - 204
EP - 206
JO - Journal of Structural Biology
JF - Journal of Structural Biology
SN - 1047-8477
IS - 2
ER -