Abstract
Δ1-Tetrahydrocannabinolic acid (THCA) synthase is a novel oxidoreductase that catalyzes the biosynthesis of the psychoactive compound THCA in Cannabis sativa (Mexican strain). In order to investigate the structure-function relationship of THCA synthase, this enzyme was overproduced in insect cells, purified and finally crystallized in 0.1 M HEPES buffer pH 7.5 containing 1.4 M sodium citrate. A single crystal suitable for X-ray diffraction measurement was obtained in 0.09 M HEPES buffer pH 7.5 containing 1.26 M sodium citrate. The crystal diffracted to 2.7 Å resolution at beamline BL41XU, SPring-8. The crystal belonged to the primitive cubic space group P432, with unit-cell parameters a = b = c = 178.2 Å. The calculated Matthews coefficient was approximately 4.1 or 2.0 Å3 Da-1 assuming the presence of one or two molecules of THCA synthase in the asymmetric unit, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 799-801 |
| Number of pages | 3 |
| Journal | Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
| Volume | 61 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Aug 1 2005 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Structural Biology
- Biochemistry
- Genetics
- Condensed Matter Physics
Cite this
Crystallization of Δ1-tetrahydrocannabinolic acid (THCA) synthase from Cannabis sativa. / Shoyama, Yoshinari; Takeuchi, Ayako; Taura, Futoshi; Tamada, Taro; Adachi, Motoyasu; Kuroki, Ryota; Shoyama, Yukihiro; Morimoto, Satoshi.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 61, No. 8, 01.08.2005, p. 799-801.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Crystallization of Δ1-tetrahydrocannabinolic acid (THCA) synthase from Cannabis sativa
AU - Shoyama, Yoshinari
AU - Takeuchi, Ayako
AU - Taura, Futoshi
AU - Tamada, Taro
AU - Adachi, Motoyasu
AU - Kuroki, Ryota
AU - Shoyama, Yukihiro
AU - Morimoto, Satoshi
PY - 2005/8/1
Y1 - 2005/8/1
N2 - Δ1-Tetrahydrocannabinolic acid (THCA) synthase is a novel oxidoreductase that catalyzes the biosynthesis of the psychoactive compound THCA in Cannabis sativa (Mexican strain). In order to investigate the structure-function relationship of THCA synthase, this enzyme was overproduced in insect cells, purified and finally crystallized in 0.1 M HEPES buffer pH 7.5 containing 1.4 M sodium citrate. A single crystal suitable for X-ray diffraction measurement was obtained in 0.09 M HEPES buffer pH 7.5 containing 1.26 M sodium citrate. The crystal diffracted to 2.7 Å resolution at beamline BL41XU, SPring-8. The crystal belonged to the primitive cubic space group P432, with unit-cell parameters a = b = c = 178.2 Å. The calculated Matthews coefficient was approximately 4.1 or 2.0 Å3 Da-1 assuming the presence of one or two molecules of THCA synthase in the asymmetric unit, respectively.
AB - Δ1-Tetrahydrocannabinolic acid (THCA) synthase is a novel oxidoreductase that catalyzes the biosynthesis of the psychoactive compound THCA in Cannabis sativa (Mexican strain). In order to investigate the structure-function relationship of THCA synthase, this enzyme was overproduced in insect cells, purified and finally crystallized in 0.1 M HEPES buffer pH 7.5 containing 1.4 M sodium citrate. A single crystal suitable for X-ray diffraction measurement was obtained in 0.09 M HEPES buffer pH 7.5 containing 1.26 M sodium citrate. The crystal diffracted to 2.7 Å resolution at beamline BL41XU, SPring-8. The crystal belonged to the primitive cubic space group P432, with unit-cell parameters a = b = c = 178.2 Å. The calculated Matthews coefficient was approximately 4.1 or 2.0 Å3 Da-1 assuming the presence of one or two molecules of THCA synthase in the asymmetric unit, respectively.
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UR - http://www.scopus.com/inward/citedby.url?scp=33747062736&partnerID=8YFLogxK
U2 - 10.1107/S1744309105023365
DO - 10.1107/S1744309105023365
M3 - Article
C2 - 16511162
AN - SCOPUS:33747062736
VL - 61
SP - 799
EP - 801
JO - Acta Crystallographica Section F:Structural Biology Communications
JF - Acta Crystallographica Section F:Structural Biology Communications
SN - 1744-3091
IS - 8
ER -