Crystallization of Δ1-tetrahydrocannabinolic acid (THCA) synthase from Cannabis sativa

Yoshinari Shoyama, Ayako Takeuchi, Futoshi Taura, Taro Tamada, Motoyasu Adachi, Ryota Kuroki, Yukihiro Shoyama, Satoshi Morimoto

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Δ1-Tetrahydrocannabinolic acid (THCA) synthase is a novel oxidoreductase that catalyzes the biosynthesis of the psychoactive compound THCA in Cannabis sativa (Mexican strain). In order to investigate the structure-function relationship of THCA synthase, this enzyme was overproduced in insect cells, purified and finally crystallized in 0.1 M HEPES buffer pH 7.5 containing 1.4 M sodium citrate. A single crystal suitable for X-ray diffraction measurement was obtained in 0.09 M HEPES buffer pH 7.5 containing 1.26 M sodium citrate. The crystal diffracted to 2.7 Å resolution at beamline BL41XU, SPring-8. The crystal belonged to the primitive cubic space group P432, with unit-cell parameters a = b = c = 178.2 Å. The calculated Matthews coefficient was approximately 4.1 or 2.0 Å3 Da-1 assuming the presence of one or two molecules of THCA synthase in the asymmetric unit, respectively.

Original languageEnglish
Pages (from-to)799-801
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume61
Issue number8
DOIs
Publication statusPublished - Aug 1 2005

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Cannabis
Crystallization
crystallization
HEPES
acids
Acids
citrates
Buffers
buffers
sodium
Crystals
biosynthesis
insects
Biosynthesis
cells
X-Ray Diffraction
crystals
Insects
enzymes
Oxidoreductases

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

Crystallization of Δ1-tetrahydrocannabinolic acid (THCA) synthase from Cannabis sativa. / Shoyama, Yoshinari; Takeuchi, Ayako; Taura, Futoshi; Tamada, Taro; Adachi, Motoyasu; Kuroki, Ryota; Shoyama, Yukihiro; Morimoto, Satoshi.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 61, No. 8, 01.08.2005, p. 799-801.

Research output: Contribution to journalArticle

Shoyama, Yoshinari ; Takeuchi, Ayako ; Taura, Futoshi ; Tamada, Taro ; Adachi, Motoyasu ; Kuroki, Ryota ; Shoyama, Yukihiro ; Morimoto, Satoshi. / Crystallization of Δ1-tetrahydrocannabinolic acid (THCA) synthase from Cannabis sativa. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2005 ; Vol. 61, No. 8. pp. 799-801.
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AU - Adachi, Motoyasu

AU - Kuroki, Ryota

AU - Shoyama, Yukihiro

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