Crystallization of Human Erythrocyte Band 3, the anion exchanger, at the International Space Station “KIBO”

Hinako Hatae, Koji Inaka, Ryo Okamura, Naoki Furubayashi, Masayuki Kamo, Takuya Kobayashi, Yoshito Abe, So Iwata, Naotaka Hamasaki

Research output: Contribution to journalArticle

Abstract

Band 3 mediates the Cl and HCO3 exchange across the red blood cell membrane and plays a pivotal role for delivering oxygen appropriately to metabolically active tissues. For understanding molecular mechanisms, it is essential to know the structure and function relationship. In terrestrial environments, however, nobody could make good quality crystals of Band 3 for the X-ray crystallographic study. In this study, we purified the transmembrane domain of Band 3 from human red blood cells and crystallized the purified Band 3 without the Fab fragment at the International Space Station “KIBO” under microgravity environments.

Original languageEnglish
Pages (from-to)91-93
Number of pages3
JournalAnalytical Biochemistry
Volume559
DOIs
Publication statusPublished - Oct 15 2018

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Chloride-Bicarbonate Antiporters
Space stations
Crystallization
Blood
Erythrocytes
Weightlessness
Immunoglobulin Fab Fragments
Microgravity
Cell membranes
Cells
Cell Membrane
X-Rays
Tissue
Oxygen
X rays
Crystals

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Hatae, H., Inaka, K., Okamura, R., Furubayashi, N., Kamo, M., Kobayashi, T., ... Hamasaki, N. (2018). Crystallization of Human Erythrocyte Band 3, the anion exchanger, at the International Space Station “KIBO”. Analytical Biochemistry, 559, 91-93. https://doi.org/10.1016/j.ab.2018.08.009

Crystallization of Human Erythrocyte Band 3, the anion exchanger, at the International Space Station “KIBO”. / Hatae, Hinako; Inaka, Koji; Okamura, Ryo; Furubayashi, Naoki; Kamo, Masayuki; Kobayashi, Takuya; Abe, Yoshito; Iwata, So; Hamasaki, Naotaka.

In: Analytical Biochemistry, Vol. 559, 15.10.2018, p. 91-93.

Research output: Contribution to journalArticle

Hatae, H, Inaka, K, Okamura, R, Furubayashi, N, Kamo, M, Kobayashi, T, Abe, Y, Iwata, S & Hamasaki, N 2018, 'Crystallization of Human Erythrocyte Band 3, the anion exchanger, at the International Space Station “KIBO”', Analytical Biochemistry, vol. 559, pp. 91-93. https://doi.org/10.1016/j.ab.2018.08.009
Hatae, Hinako ; Inaka, Koji ; Okamura, Ryo ; Furubayashi, Naoki ; Kamo, Masayuki ; Kobayashi, Takuya ; Abe, Yoshito ; Iwata, So ; Hamasaki, Naotaka. / Crystallization of Human Erythrocyte Band 3, the anion exchanger, at the International Space Station “KIBO”. In: Analytical Biochemistry. 2018 ; Vol. 559. pp. 91-93.
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