Crystallization of Human Erythrocyte Band 3, the anion exchanger, at the International Space Station “KIBO”

Hinako Hatae, Koji Inaka, Ryo Okamura, Naoki Furubayashi, Masayuki Kamo, Takuya Kobayashi, Yoshito Abe, So Iwata, Naotaka Hamasaki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Band 3 mediates the Cl and HCO3 exchange across the red blood cell membrane and plays a pivotal role for delivering oxygen appropriately to metabolically active tissues. For understanding molecular mechanisms, it is essential to know the structure and function relationship. In terrestrial environments, however, nobody could make good quality crystals of Band 3 for the X-ray crystallographic study. In this study, we purified the transmembrane domain of Band 3 from human red blood cells and crystallized the purified Band 3 without the Fab fragment at the International Space Station “KIBO” under microgravity environments.

Original languageEnglish
Pages (from-to)91-93
Number of pages3
JournalAnalytical Biochemistry
Volume559
DOIs
Publication statusPublished - Oct 15 2018

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Hatae, H., Inaka, K., Okamura, R., Furubayashi, N., Kamo, M., Kobayashi, T., Abe, Y., Iwata, S., & Hamasaki, N. (2018). Crystallization of Human Erythrocyte Band 3, the anion exchanger, at the International Space Station “KIBO”. Analytical Biochemistry, 559, 91-93. https://doi.org/10.1016/j.ab.2018.08.009