Crystallographic and functional studies of very short patch repair endonuclease

Susan E. Tsutakawa, Takanori Muto, Tomohiko Kawate, Hisato Jingami, Naoki Kunishima, Mariko Ariyoshi, Daisuke Kohda, Masako Nakagawa, Kosuke Morikawa

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Vsr endonuclease plays a crucial role in the repair of TG mismatched base pairs, which are generated by the spontaneous degradation of methylated cytidines; Vsr recognizes the mismatched base pair and cleaves the phosphate backbone 5' to the thymidine. We have determined the crystal structure of a truncated form of this endonuclease at 1.8 Å resolution. The protein contains one structural zinc-binding module. Unexpectedly, its overall topology resembles members of the type II restriction endonuclease family. Subsequent mutational and biochemical analyses showed that certain elements in the catalytic site are also conserved. However, the identification of a critical histidine and evidence of an active site metal-binding coordination that is novel to endonucleases indicate a distinct catalytic mechanism.

Original languageEnglish
Pages (from-to)621-628
Number of pages8
JournalMolecular Cell
Volume3
Issue number5
DOIs
Publication statusPublished - Jan 1 1999
Externally publishedYes

Fingerprint

Endonucleases
Base Pairing
Catalytic Domain
Type II Site Specific Deoxyribonucleases
Cytidine
Histidine
Thymidine
Zinc
Metals
Phosphates
Proteins
vsr endonuclease

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Tsutakawa, S. E., Muto, T., Kawate, T., Jingami, H., Kunishima, N., Ariyoshi, M., ... Morikawa, K. (1999). Crystallographic and functional studies of very short patch repair endonuclease. Molecular Cell, 3(5), 621-628. https://doi.org/10.1016/S1097-2765(00)80355-X

Crystallographic and functional studies of very short patch repair endonuclease. / Tsutakawa, Susan E.; Muto, Takanori; Kawate, Tomohiko; Jingami, Hisato; Kunishima, Naoki; Ariyoshi, Mariko; Kohda, Daisuke; Nakagawa, Masako; Morikawa, Kosuke.

In: Molecular Cell, Vol. 3, No. 5, 01.01.1999, p. 621-628.

Research output: Contribution to journalArticle

Tsutakawa, SE, Muto, T, Kawate, T, Jingami, H, Kunishima, N, Ariyoshi, M, Kohda, D, Nakagawa, M & Morikawa, K 1999, 'Crystallographic and functional studies of very short patch repair endonuclease', Molecular Cell, vol. 3, no. 5, pp. 621-628. https://doi.org/10.1016/S1097-2765(00)80355-X
Tsutakawa SE, Muto T, Kawate T, Jingami H, Kunishima N, Ariyoshi M et al. Crystallographic and functional studies of very short patch repair endonuclease. Molecular Cell. 1999 Jan 1;3(5):621-628. https://doi.org/10.1016/S1097-2765(00)80355-X
Tsutakawa, Susan E. ; Muto, Takanori ; Kawate, Tomohiko ; Jingami, Hisato ; Kunishima, Naoki ; Ariyoshi, Mariko ; Kohda, Daisuke ; Nakagawa, Masako ; Morikawa, Kosuke. / Crystallographic and functional studies of very short patch repair endonuclease. In: Molecular Cell. 1999 ; Vol. 3, No. 5. pp. 621-628.
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