CUE domain-containing protein vps901 is required for vacuolar protein transport in Schizosaccharomyces pombe

Mai Nakase, Yuta Tsukamoto, Akira Hosomi, Tomo Matsuda, Masaaki Miyamoto, Kaoru Takegawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The functions of two Schizosaccharomyces pombe Vps9- like genes, SPBC4F6.10/vps901+ and SPBC29A10.11c/ vps902+, were characterized. Genomic sequence analysis predicted that Vps901p contains a VPS9 domain, whereas cDNA analyses revealed that Vps901p contains a CUE domain (coupling of ubiquitin to ER degradation) in its C-terminal region. Deletion of vps901+ resulted in mis-sorting and secretion of S. pombe vacuolar carboxypeptidase Cpy1p, whereas deletion of vps902+ had no effect, suggesting that only Vps901p functions in vacuolar protein transport in S. pombe. Deletion of vps901+ further produced pleiotropic phenotypes, including vacuolar homotypic fusion and endocytosis defects. Heterologous expression of the budding yeast VPS9 gene corrected the CPY mis-sorting defect in vps901Δ cells. These findings suggest that the VPS9 domain of Vps901p is required for vacuolar protein trafficking in S. pombe.

Original languageEnglish
Pages (from-to)652-659
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number4
DOIs
Publication statusPublished - Apr 30 2012

Fingerprint

Schizosaccharomyces
Protein Transport
Sorting
Genes
Carboxypeptidases
Defects
Ubiquitin
Yeast
Proteins
Fusion reactions
Complementary DNA
Degradation
Saccharomycetales
Endocytosis
Sequence Analysis
Phenotype
Protein Domains

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

CUE domain-containing protein vps901 is required for vacuolar protein transport in Schizosaccharomyces pombe. / Nakase, Mai; Tsukamoto, Yuta; Hosomi, Akira; Matsuda, Tomo; Miyamoto, Masaaki; Takegawa, Kaoru.

In: Bioscience, Biotechnology and Biochemistry, Vol. 76, No. 4, 30.04.2012, p. 652-659.

Research output: Contribution to journalArticle

Nakase, Mai ; Tsukamoto, Yuta ; Hosomi, Akira ; Matsuda, Tomo ; Miyamoto, Masaaki ; Takegawa, Kaoru. / CUE domain-containing protein vps901 is required for vacuolar protein transport in Schizosaccharomyces pombe. In: Bioscience, Biotechnology and Biochemistry. 2012 ; Vol. 76, No. 4. pp. 652-659.
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