Culture-negative brain abscess with Streptococcus intermedius infection with diagnosis established by direct nucleotide sequence analysis of the 16s ribosomal RNA gene

Naoko Saito, Ayumi Hida, Yuri Koide, Tadasuke Ooka, Yaeko Ichikawa, Jun Shimizu, Akitake Mukasa, Hirofumi Nakatomi, Shuji Hatakeyama, Tetsuya Hayashi, Shoji Tsuji

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

A 70-year-old woman developed a headache for a month followed by right upper limb weakness. CT scan and MRI showed multiple ring-enhancing lesions. An intracerebral aspiration of an abscess was performed, but culture results were negative. The nucleotide sequence analysis of the 16S rRNA gene from the specimens identified Streptococcus intermedius. Given this result, S. intermedius was cultured by enrichment culture, and its sensitivities to antibiotics were determined. The patient exhibited complete remission. Thus, 16S rRNA gene analysis was highly useful not only for pathogen identification with negative culture results but also for the appropriate selection of antibiotics.

Original languageEnglish
Pages (from-to)211-216
Number of pages6
JournalInternal Medicine
Volume51
Issue number2
DOIs
Publication statusPublished - 2012

All Science Journal Classification (ASJC) codes

  • Internal Medicine

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