Curved EFC/F-BAR-Domain Dimers Are Joined End to End into a Filament for Membrane Invagination in Endocytosis

Atsushi Shimada; Hideaki Niwa; Kazuya Tsujita; Shiro Suetsugu; Koji Nitta; Kyoko Hanawa-Suetsugu; Ryogo Akasaka; Yuri Nishino; Mitsutoshi Toyama; Lirong Chen; Zhi Jie Liu; Bi Cheng Wang; Masaki Yamamoto; Takaho Terada; Atsuo Miyazawa; Akiko Tanaka; Sumio Sugano; Mikako Shirouzu; Kuniaki Nagayama; Tadaomi Takenawa; Shigeyuki Yokoyama

doi:10.1016/j.cell.2007.03.040

Curved EFC/F-BAR-Domain Dimers Are Joined End to End into a Filament for Membrane Invagination in Endocytosis

Atsushi Shimada, Hideaki Niwa, Kazuya Tsujita, Shiro Suetsugu, Koji Nitta, Kyoko Hanawa-Suetsugu, Ryogo Akasaka, Yuri Nishino, Mitsutoshi Toyama, Lirong Chen, Zhi Jie Liu, Bi Cheng Wang, Masaki Yamamoto, Takaho Terada, Atsuo Miyazawa, Akiko Tanaka, Sumio Sugano, Mikako Shirouzu, Kuniaki Nagayama, Tadaomi TakenawaShigeyuki Yokoyama

Research output: Contribution to journal › Article › peer-review

303 Citations (Scopus)

Abstract

Pombe Cdc15 homology (PCH) proteins play an important role in a variety of actin-based processes, including clathrin-mediated endocytosis (CME). The defining feature of the PCH proteins is an evolutionarily conserved EFC/F-BAR domain for membrane association and tubulation. In the present study, we solved the crystal structures of the EFC domains of human FBP17 and CIP4. The structures revealed a gently curved helical-bundle dimer of ∼220 Å in length, which forms filaments through end-to-end interactions in the crystals. The curved EFC dimer fits a tubular membrane with an ∼600 Å diameter. We subsequently proposed a model in which the curved EFC filament drives tubulation. In fact, striation of tubular membranes was observed by phase-contrast cryo-transmission electron microscopy, and mutations that impaired filament formation also impaired membrane tubulation and cell membrane invagination. Furthermore, FBP17 is recruited to clathrin-coated pits in the late stage of CME, indicating its physiological role.

Original language	English
Pages (from-to)	761-772
Number of pages	12
Journal	Cell
Volume	129
Issue number	4
DOIs	https://doi.org/10.1016/j.cell.2007.03.040
Publication status	Published - May 18 2007
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

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Shimada, A., Niwa, H., Tsujita, K., Suetsugu, S., Nitta, K., Hanawa-Suetsugu, K., Akasaka, R., Nishino, Y., Toyama, M., Chen, L., Liu, Z. J., Wang, B. C., Yamamoto, M., Terada, T., Miyazawa, A., Tanaka, A., Sugano, S., Shirouzu, M., Nagayama, K., ... Yokoyama, S. (2007). Curved EFC/F-BAR-Domain Dimers Are Joined End to End into a Filament for Membrane Invagination in Endocytosis. Cell, 129(4), 761-772. https://doi.org/10.1016/j.cell.2007.03.040

Curved EFC/F-BAR-Domain Dimers Are Joined End to End into a Filament for Membrane Invagination in Endocytosis. / Shimada, Atsushi; Niwa, Hideaki; Tsujita, Kazuya; Suetsugu, Shiro; Nitta, Koji; Hanawa-Suetsugu, Kyoko; Akasaka, Ryogo; Nishino, Yuri; Toyama, Mitsutoshi; Chen, Lirong; Liu, Zhi Jie; Wang, Bi Cheng; Yamamoto, Masaki; Terada, Takaho; Miyazawa, Atsuo; Tanaka, Akiko; Sugano, Sumio; Shirouzu, Mikako; Nagayama, Kuniaki; Takenawa, Tadaomi; Yokoyama, Shigeyuki.

In: Cell, Vol. 129, No. 4, 18.05.2007, p. 761-772.

Research output: Contribution to journal › Article › peer-review

Shimada, A, Niwa, H, Tsujita, K, Suetsugu, S, Nitta, K, Hanawa-Suetsugu, K, Akasaka, R, Nishino, Y, Toyama, M, Chen, L, Liu, ZJ, Wang, BC, Yamamoto, M, Terada, T, Miyazawa, A, Tanaka, A, Sugano, S, Shirouzu, M, Nagayama, K, Takenawa, T & Yokoyama, S 2007, 'Curved EFC/F-BAR-Domain Dimers Are Joined End to End into a Filament for Membrane Invagination in Endocytosis', Cell, vol. 129, no. 4, pp. 761-772. https://doi.org/10.1016/j.cell.2007.03.040

Shimada, Atsushi ; Niwa, Hideaki ; Tsujita, Kazuya ; Suetsugu, Shiro ; Nitta, Koji ; Hanawa-Suetsugu, Kyoko ; Akasaka, Ryogo ; Nishino, Yuri ; Toyama, Mitsutoshi ; Chen, Lirong ; Liu, Zhi Jie ; Wang, Bi Cheng ; Yamamoto, Masaki ; Terada, Takaho ; Miyazawa, Atsuo ; Tanaka, Akiko ; Sugano, Sumio ; Shirouzu, Mikako ; Nagayama, Kuniaki ; Takenawa, Tadaomi ; Yokoyama, Shigeyuki. / Curved EFC/F-BAR-Domain Dimers Are Joined End to End into a Filament for Membrane Invagination in Endocytosis. In: Cell. 2007 ; Vol. 129, No. 4. pp. 761-772.

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abstract = "Pombe Cdc15 homology (PCH) proteins play an important role in a variety of actin-based processes, including clathrin-mediated endocytosis (CME). The defining feature of the PCH proteins is an evolutionarily conserved EFC/F-BAR domain for membrane association and tubulation. In the present study, we solved the crystal structures of the EFC domains of human FBP17 and CIP4. The structures revealed a gently curved helical-bundle dimer of ∼220 {\AA} in length, which forms filaments through end-to-end interactions in the crystals. The curved EFC dimer fits a tubular membrane with an ∼600 {\AA} diameter. We subsequently proposed a model in which the curved EFC filament drives tubulation. In fact, striation of tubular membranes was observed by phase-contrast cryo-transmission electron microscopy, and mutations that impaired filament formation also impaired membrane tubulation and cell membrane invagination. Furthermore, FBP17 is recruited to clathrin-coated pits in the late stage of CME, indicating its physiological role.",

author = "Atsushi Shimada and Hideaki Niwa and Kazuya Tsujita and Shiro Suetsugu and Koji Nitta and Kyoko Hanawa-Suetsugu and Ryogo Akasaka and Yuri Nishino and Mitsutoshi Toyama and Lirong Chen and Liu, {Zhi Jie} and Wang, {Bi Cheng} and Masaki Yamamoto and Takaho Terada and Atsuo Miyazawa and Akiko Tanaka and Sumio Sugano and Mikako Shirouzu and Kuniaki Nagayama and Tadaomi Takenawa and Shigeyuki Yokoyama",

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AU - Niwa, Hideaki

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AU - Suetsugu, Shiro

AU - Nitta, Koji

AU - Hanawa-Suetsugu, Kyoko

AU - Akasaka, Ryogo

AU - Nishino, Yuri

AU - Toyama, Mitsutoshi

AU - Chen, Lirong

AU - Liu, Zhi Jie

AU - Wang, Bi Cheng

AU - Yamamoto, Masaki

AU - Terada, Takaho

AU - Miyazawa, Atsuo

AU - Tanaka, Akiko

AU - Sugano, Sumio

AU - Shirouzu, Mikako

AU - Nagayama, Kuniaki

AU - Takenawa, Tadaomi

AU - Yokoyama, Shigeyuki

PY - 2007/5/18

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N2 - Pombe Cdc15 homology (PCH) proteins play an important role in a variety of actin-based processes, including clathrin-mediated endocytosis (CME). The defining feature of the PCH proteins is an evolutionarily conserved EFC/F-BAR domain for membrane association and tubulation. In the present study, we solved the crystal structures of the EFC domains of human FBP17 and CIP4. The structures revealed a gently curved helical-bundle dimer of ∼220 Å in length, which forms filaments through end-to-end interactions in the crystals. The curved EFC dimer fits a tubular membrane with an ∼600 Å diameter. We subsequently proposed a model in which the curved EFC filament drives tubulation. In fact, striation of tubular membranes was observed by phase-contrast cryo-transmission electron microscopy, and mutations that impaired filament formation also impaired membrane tubulation and cell membrane invagination. Furthermore, FBP17 is recruited to clathrin-coated pits in the late stage of CME, indicating its physiological role.

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