Cutting edge: Dominant effect of Ile50Val variant of the human IL-4 receptor α-chain in IgE synthesis

Hiromichi Mitsuyasu, Yukiyoshi Yanagihara, Xiao Quan Mao, Pei Sun Gao, Yojiro Arinobu, Kenji Ihara, Akira Takabayashi, Toshiro Hara, Tadao Enomoto, Sei Sasaki, Minoru Kawai, Naotaka Hamasaki, Taro Shirakawa, Julian M. Hopkin, Kenji Izuhara

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159 Citations (Scopus)

Abstract

Two variant of the IL-4R α-chain (IL-4Rα) gene have been recently identified in association with different atopic disorders. To clarify the etiological relationship between the two variants, we analyzed responsiveness to IL-4 of transfectants with four kinds of IL-4Rα carrying either Val or Ile at 50 and either Gln or Arg at 551. The substitution of Ile for Val augmented STAT6 activation, proliferation, and transcription activity of the Iε promoter by IL-4, whereas that of Arg for Gln did not change these IL-4 signals. Arg551 was not associated with atopic asthma in the Japanese population. CD23 expression and IgE synthesis by IL-4 were augmented in Ile50-bearing PBMC, compared with those bearing Val50. Taken together, substitution of Arg551 does not enhance the IL-4 signal for generation of germline ε transcript, whereas the substitution of Ile50 contributes to enhancement of IgE synthesis.

Original languageEnglish
Pages (from-to)1227-1231
Number of pages5
JournalJournal of Immunology
Volume162
Issue number3
Publication statusPublished - Feb 1 1999

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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    Mitsuyasu, H., Yanagihara, Y., Mao, X. Q., Gao, P. S., Arinobu, Y., Ihara, K., Takabayashi, A., Hara, T., Enomoto, T., Sasaki, S., Kawai, M., Hamasaki, N., Shirakawa, T., Hopkin, J. M., & Izuhara, K. (1999). Cutting edge: Dominant effect of Ile50Val variant of the human IL-4 receptor α-chain in IgE synthesis. Journal of Immunology, 162(3), 1227-1231.