ヘアリーベッチにおけるシアナミドの生合成部位

Translated title of the contribution: Cyanamide-biosynthesizing organ in hairy vetch Vicia villosa subsp. varia

笠原 良平, 加茂 綱嗣, 廣田 満, 平舘 俊太郎, 山谷 紘子, 藤井 義晴

Research output: Contribution to journalArticle

Abstract

Cyanamide (NH_2CN) has been isolated from hairy vetch Vicia villosa subsp. varia as a natural product for the first time. We have already clarified that the cyanamide content in hairy vetch seedlings cultivated in the growth chamber reaches its maximum at 3-4 week. The present study aims at elucidating the organ in which cyanamide is biosynthesized. The cyanamide contents in the leaves, the stems, and the roots of 4-week-old seedlings were 629 ± 209, 256 ± 134, and < 20 μg/gFW (average ± SD), respectively. This suggests that cyanamide is biosynthesized in the aerial parts (the leaves plus the stems). To investigate the cyanamide-biosynthesizing organ, we compared the ^^<15>N incorporation into cyanamide in the whole aerial parts to that in the apical portion including the younger leaves. The ^^<15>N isotope abundance ratio in the isolated cyanamide was 3.99 ± 0.61% (average ± SE) when the whole aerial parts was incubated with ^^<15>N-labeled nitrogen sources, which was significantly higher than that of control (0.28 ± 0.03%). In contrast, the cyanamide isolated from the apical portion was not ^^<15>N-labeled on the same condition. These results imply that cyanamide is biosynthesized in the mature leaves.
Original languageJapanese
Pages (from-to)81
Journal植物の生長調節 = Regulation of plant growth & development
Volume42
Publication statusPublished - Oct 5 2007

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