TY - JOUR
T1 - Cyanobacterial production of 1,3-propanediol directly from carbon dioxide using a synthetic metabolic pathway
AU - Hirokawa, Yasutaka
AU - Maki, Yuki
AU - Tatsuke, Tsuneyuki
AU - Hanai, Taizo
N1 - Funding Information:
This study was financially supported by the Core Research of Evolutional Science & Technology program (CREST) from the Japan Science and Technology Agency (JST) . Appendix A
Publisher Copyright:
© 2016 International Metabolic Engineering Society.
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Production of chemicals directly from carbon dioxide using light energy is an attractive option for a sustainable future. The 1,3-propanediol (1,3-PDO) production directly from carbon dioxide was achieved by engineered Synechococcus elongatus PCC 7942 with a synthetic metabolic pathway. Glycerol dehydratase catalyzing the conversion of glycerol to 3-hydroxypropionaldehyde in a coenzyme B12-dependent manner worked in S. elongatus PCC 7942 without addition of vitamin B12, suggesting that the intrinsic pseudovitamin B12 served as a substitute of coenzyme B12. The highest titers of 1,3-PDO (3.79±0.23mM; 288±17.7mg/L) and glycerol (12.62±1.55mM; 1.16±0.14g/L), precursor of 1,3-PDO, were reached after 14 days of culture under optimized conditions in this study.
AB - Production of chemicals directly from carbon dioxide using light energy is an attractive option for a sustainable future. The 1,3-propanediol (1,3-PDO) production directly from carbon dioxide was achieved by engineered Synechococcus elongatus PCC 7942 with a synthetic metabolic pathway. Glycerol dehydratase catalyzing the conversion of glycerol to 3-hydroxypropionaldehyde in a coenzyme B12-dependent manner worked in S. elongatus PCC 7942 without addition of vitamin B12, suggesting that the intrinsic pseudovitamin B12 served as a substitute of coenzyme B12. The highest titers of 1,3-PDO (3.79±0.23mM; 288±17.7mg/L) and glycerol (12.62±1.55mM; 1.16±0.14g/L), precursor of 1,3-PDO, were reached after 14 days of culture under optimized conditions in this study.
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U2 - 10.1016/j.ymben.2015.12.008
DO - 10.1016/j.ymben.2015.12.008
M3 - Article
C2 - 26769097
AN - SCOPUS:84954288565
SN - 1096-7176
VL - 34
SP - 97
EP - 103
JO - Metabolic Engineering
JF - Metabolic Engineering
ER -