Cyanobacterial production of 1,3-propanediol directly from carbon dioxide using a synthetic metabolic pathway

Yasutaka Hirokawa, Yuki Maki, Tsuneyuki Tatsuke, Taizo Hanai

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Production of chemicals directly from carbon dioxide using light energy is an attractive option for a sustainable future. The 1,3-propanediol (1,3-PDO) production directly from carbon dioxide was achieved by engineered Synechococcus elongatus PCC 7942 with a synthetic metabolic pathway. Glycerol dehydratase catalyzing the conversion of glycerol to 3-hydroxypropionaldehyde in a coenzyme B12-dependent manner worked in S. elongatus PCC 7942 without addition of vitamin B12, suggesting that the intrinsic pseudovitamin B12 served as a substitute of coenzyme B12. The highest titers of 1,3-PDO (3.79±0.23mM; 288±17.7mg/L) and glycerol (12.62±1.55mM; 1.16±0.14g/L), precursor of 1,3-PDO, were reached after 14 days of culture under optimized conditions in this study.

Original languageEnglish
Pages (from-to)97-103
Number of pages7
JournalMetabolic Engineering
Volume34
DOIs
Publication statusPublished - Mar 1 2016

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Cyanobacterial production of 1,3-propanediol directly from carbon dioxide using a synthetic metabolic pathway'. Together they form a unique fingerprint.

  • Cite this