Cyclin A Is Redundant in Fibroblasts but Essential in Hematopoietic and Embryonic Stem Cells

Ilona Kalaszczynska, Yan Geng, Tadafumi Iino, Shin ichi Mizuno, Yoon Choi, Ilona Kondratiuk, Daniel P. Silver, Debra J. Wolgemuth, Koichi Akashi, Piotr Sicinski

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Cyclins are regulatory subunits of cyclin-dependent kinases. Cyclin A, the first cyclin ever cloned, is thought to be an essential component of the cell-cycle engine. Mammalian cells encode two A-type cyclins, testis-specific cyclin A1 and ubiquitously expressed cyclin A2. Here, we tested the requirement for cyclin A function using conditional knockout mice lacking both A-type cyclins. We found that acute ablation of cyclin A in fibroblasts did not affect cell proliferation, but led to prolonged expression of another cyclin, cyclin E, across the cell cycle. However, combined ablation of all A- and E-type cyclins extinguished cell division. In contrast, cyclin A function was essential for cell-cycle progression of hematopoietic and embryonic stem cells. Expression of cyclin A is particularly high in these compartments, which might render stem cells dependent on cyclin A, whereas in fibroblasts cyclins A and E play redundant roles in cell proliferation.

Original languageEnglish
Pages (from-to)352-365
Number of pages14
JournalCell
Volume138
Issue number2
DOIs
Publication statusPublished - Jul 23 2009

Fingerprint

Cyclin A
Fibroblasts
Embryonic Stem Cells
Hematopoietic Stem Cells
Stem cells
Cyclin E
Cells
Cyclins
Cell Cycle
Cell proliferation
Ablation
Cyclin A1
Cyclin A2
Cell Proliferation
Cyclin-Dependent Kinases
Knockout Mice
Cell Division
Testis
Stem Cells
Engines

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kalaszczynska, I., Geng, Y., Iino, T., Mizuno, S. I., Choi, Y., Kondratiuk, I., ... Sicinski, P. (2009). Cyclin A Is Redundant in Fibroblasts but Essential in Hematopoietic and Embryonic Stem Cells. Cell, 138(2), 352-365. https://doi.org/10.1016/j.cell.2009.04.062

Cyclin A Is Redundant in Fibroblasts but Essential in Hematopoietic and Embryonic Stem Cells. / Kalaszczynska, Ilona; Geng, Yan; Iino, Tadafumi; Mizuno, Shin ichi; Choi, Yoon; Kondratiuk, Ilona; Silver, Daniel P.; Wolgemuth, Debra J.; Akashi, Koichi; Sicinski, Piotr.

In: Cell, Vol. 138, No. 2, 23.07.2009, p. 352-365.

Research output: Contribution to journalArticle

Kalaszczynska, I, Geng, Y, Iino, T, Mizuno, SI, Choi, Y, Kondratiuk, I, Silver, DP, Wolgemuth, DJ, Akashi, K & Sicinski, P 2009, 'Cyclin A Is Redundant in Fibroblasts but Essential in Hematopoietic and Embryonic Stem Cells', Cell, vol. 138, no. 2, pp. 352-365. https://doi.org/10.1016/j.cell.2009.04.062
Kalaszczynska I, Geng Y, Iino T, Mizuno SI, Choi Y, Kondratiuk I et al. Cyclin A Is Redundant in Fibroblasts but Essential in Hematopoietic and Embryonic Stem Cells. Cell. 2009 Jul 23;138(2):352-365. https://doi.org/10.1016/j.cell.2009.04.062
Kalaszczynska, Ilona ; Geng, Yan ; Iino, Tadafumi ; Mizuno, Shin ichi ; Choi, Yoon ; Kondratiuk, Ilona ; Silver, Daniel P. ; Wolgemuth, Debra J. ; Akashi, Koichi ; Sicinski, Piotr. / Cyclin A Is Redundant in Fibroblasts but Essential in Hematopoietic and Embryonic Stem Cells. In: Cell. 2009 ; Vol. 138, No. 2. pp. 352-365.
@article{cb32bfb7e54d4a6b8f736713f9170601,
title = "Cyclin A Is Redundant in Fibroblasts but Essential in Hematopoietic and Embryonic Stem Cells",
abstract = "Cyclins are regulatory subunits of cyclin-dependent kinases. Cyclin A, the first cyclin ever cloned, is thought to be an essential component of the cell-cycle engine. Mammalian cells encode two A-type cyclins, testis-specific cyclin A1 and ubiquitously expressed cyclin A2. Here, we tested the requirement for cyclin A function using conditional knockout mice lacking both A-type cyclins. We found that acute ablation of cyclin A in fibroblasts did not affect cell proliferation, but led to prolonged expression of another cyclin, cyclin E, across the cell cycle. However, combined ablation of all A- and E-type cyclins extinguished cell division. In contrast, cyclin A function was essential for cell-cycle progression of hematopoietic and embryonic stem cells. Expression of cyclin A is particularly high in these compartments, which might render stem cells dependent on cyclin A, whereas in fibroblasts cyclins A and E play redundant roles in cell proliferation.",
author = "Ilona Kalaszczynska and Yan Geng and Tadafumi Iino and Mizuno, {Shin ichi} and Yoon Choi and Ilona Kondratiuk and Silver, {Daniel P.} and Wolgemuth, {Debra J.} and Koichi Akashi and Piotr Sicinski",
year = "2009",
month = "7",
day = "23",
doi = "10.1016/j.cell.2009.04.062",
language = "English",
volume = "138",
pages = "352--365",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "2",

}

TY - JOUR

T1 - Cyclin A Is Redundant in Fibroblasts but Essential in Hematopoietic and Embryonic Stem Cells

AU - Kalaszczynska, Ilona

AU - Geng, Yan

AU - Iino, Tadafumi

AU - Mizuno, Shin ichi

AU - Choi, Yoon

AU - Kondratiuk, Ilona

AU - Silver, Daniel P.

AU - Wolgemuth, Debra J.

AU - Akashi, Koichi

AU - Sicinski, Piotr

PY - 2009/7/23

Y1 - 2009/7/23

N2 - Cyclins are regulatory subunits of cyclin-dependent kinases. Cyclin A, the first cyclin ever cloned, is thought to be an essential component of the cell-cycle engine. Mammalian cells encode two A-type cyclins, testis-specific cyclin A1 and ubiquitously expressed cyclin A2. Here, we tested the requirement for cyclin A function using conditional knockout mice lacking both A-type cyclins. We found that acute ablation of cyclin A in fibroblasts did not affect cell proliferation, but led to prolonged expression of another cyclin, cyclin E, across the cell cycle. However, combined ablation of all A- and E-type cyclins extinguished cell division. In contrast, cyclin A function was essential for cell-cycle progression of hematopoietic and embryonic stem cells. Expression of cyclin A is particularly high in these compartments, which might render stem cells dependent on cyclin A, whereas in fibroblasts cyclins A and E play redundant roles in cell proliferation.

AB - Cyclins are regulatory subunits of cyclin-dependent kinases. Cyclin A, the first cyclin ever cloned, is thought to be an essential component of the cell-cycle engine. Mammalian cells encode two A-type cyclins, testis-specific cyclin A1 and ubiquitously expressed cyclin A2. Here, we tested the requirement for cyclin A function using conditional knockout mice lacking both A-type cyclins. We found that acute ablation of cyclin A in fibroblasts did not affect cell proliferation, but led to prolonged expression of another cyclin, cyclin E, across the cell cycle. However, combined ablation of all A- and E-type cyclins extinguished cell division. In contrast, cyclin A function was essential for cell-cycle progression of hematopoietic and embryonic stem cells. Expression of cyclin A is particularly high in these compartments, which might render stem cells dependent on cyclin A, whereas in fibroblasts cyclins A and E play redundant roles in cell proliferation.

UR - http://www.scopus.com/inward/record.url?scp=67650638697&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67650638697&partnerID=8YFLogxK

U2 - 10.1016/j.cell.2009.04.062

DO - 10.1016/j.cell.2009.04.062

M3 - Article

C2 - 19592082

AN - SCOPUS:67650638697

VL - 138

SP - 352

EP - 365

JO - Cell

JF - Cell

SN - 0092-8674

IS - 2

ER -