CyclinG contributes to G2/M arrest of cells in response to DNA damage

Atsushi Shimizu, Jun Ichi Nishida, Yousuke Ueoka, Kiyoko Kato, Toshirou Hachiya, Yumiko Kuriaki, Norio Wake

    Research output: Contribution to journalArticlepeer-review

    49 Citations (Scopus)

    Abstract

    To investigate regulation mechanisms of G2/M phase transition, we studied the association of cell cycle progression with p53-dependent p21/waf-1 and cyclinG expression. We used doxorubicin (DOX) and sodium butyrate (NaB) to accumulate p53 protein. DOX treatment resulted in an apparent increase of cells in the G2/M fraction, whereas NaB arrested cells at G1. P53 protein induction in response to DOX accompanied upregulation of p21/waf-1 and cyclinG expression. However, cyclinG was undetectable in NaB-treated cells. These results implied a putative association between increases in the proportion of cells accumulating in the G2/M fraction and enhanced cyclinG expression. Antisense oligo DNAs (AS) complementary to cyclinG mRNA inhibited the cyclinG protein expression induced by DOX treatment. This inhibition resulted in a marked reduction in the number of cells arrested at G2/M and accumulating at G1. A role for cyclinG in G2/M phase transition control is implied.

    Original languageEnglish
    Pages (from-to)529-533
    Number of pages5
    JournalBiochemical and Biophysical Research Communications
    Volume242
    Issue number3
    DOIs
    Publication statusPublished - Jan 26 1998

    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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