Cyclophosphamide-induced tolerance in fully allogeneic heart transplantation in mice

Akio Matsuura, Masahisa Katsuno, Yoshiko Suzuki, Toshiaki Ito, Kenzo Yasuura, Toshio Abe, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

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    Abstract

    We have established a long-lasting heart allograft tolerance in H-2- incompatible combinations by intravenous injection of 108 allogeneic spleen cells followed by the administration of 200 mg/kg of cyclophosphamide (CP). When C57BL/10 mice (B10, H-2b) were primed iv with 1 x 108 viable spleen cells from naive BALB/c mice (H-2(d)) and treated ip with 200 mg/kg CP 2 days later, BALB/c heart allografts were lasting in the recipients for as long as 8 weeks. On the other hand, B10 mice receiving either allogeneic spleen cells or CP rejected the heart allograft within 10 days after transplantation. A direct approach assessing the donor-reactive T cells for frequency and reactivity showed that the donor-reactive T cells were partially deleted and the rest were rendered unresponsive. CP-induced tolerance may be applied for the induction of antigen-specific tolerance to allogeneic heart transplantation.

    Original languageEnglish
    Pages (from-to)501-507
    Number of pages7
    JournalCellular Immunology
    Volume155
    Issue number2
    DOIs
    Publication statusPublished - Jan 1 1994

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    Homologous Transplantation
    Heart Transplantation
    Cyclophosphamide
    Spleen
    Allografts
    Transplantation Tolerance
    T-Lymphocytes
    Inbred C57BL Mouse
    Intravenous Injections
    Transplantation
    Antigens

    All Science Journal Classification (ASJC) codes

    • Immunology

    Cite this

    Matsuura, A., Katsuno, M., Suzuki, Y., Ito, T., Yasuura, K., Abe, T., & Yoshikai, Y. (1994). Cyclophosphamide-induced tolerance in fully allogeneic heart transplantation in mice. Cellular Immunology, 155(2), 501-507. https://doi.org/10.1006/cimm.1994.1142

    Cyclophosphamide-induced tolerance in fully allogeneic heart transplantation in mice. / Matsuura, Akio; Katsuno, Masahisa; Suzuki, Yoshiko; Ito, Toshiaki; Yasuura, Kenzo; Abe, Toshio; Yoshikai, Yasunobu.

    In: Cellular Immunology, Vol. 155, No. 2, 01.01.1994, p. 501-507.

    Research output: Contribution to journalArticle

    Matsuura, A, Katsuno, M, Suzuki, Y, Ito, T, Yasuura, K, Abe, T & Yoshikai, Y 1994, 'Cyclophosphamide-induced tolerance in fully allogeneic heart transplantation in mice', Cellular Immunology, vol. 155, no. 2, pp. 501-507. https://doi.org/10.1006/cimm.1994.1142
    Matsuura A, Katsuno M, Suzuki Y, Ito T, Yasuura K, Abe T et al. Cyclophosphamide-induced tolerance in fully allogeneic heart transplantation in mice. Cellular Immunology. 1994 Jan 1;155(2):501-507. https://doi.org/10.1006/cimm.1994.1142
    Matsuura, Akio ; Katsuno, Masahisa ; Suzuki, Yoshiko ; Ito, Toshiaki ; Yasuura, Kenzo ; Abe, Toshio ; Yoshikai, Yasunobu. / Cyclophosphamide-induced tolerance in fully allogeneic heart transplantation in mice. In: Cellular Immunology. 1994 ; Vol. 155, No. 2. pp. 501-507.
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