Cyclophosphamide-induced tolerance in fully allogeneic heart transplantation in mice

Akio Matsuura, Masahisa Katsuno, Yoshiko Suzuki, Toshiaki Ito, Kenzo Yasuura, Toshio Abe, Yasunobu Yoshikai

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    Abstract

    We have established a long-lasting heart allograft tolerance in H-2- incompatible combinations by intravenous injection of 108 allogeneic spleen cells followed by the administration of 200 mg/kg of cyclophosphamide (CP). When C57BL/10 mice (B10, H-2b) were primed iv with 1 x 108 viable spleen cells from naive BALB/c mice (H-2(d)) and treated ip with 200 mg/kg CP 2 days later, BALB/c heart allografts were lasting in the recipients for as long as 8 weeks. On the other hand, B10 mice receiving either allogeneic spleen cells or CP rejected the heart allograft within 10 days after transplantation. A direct approach assessing the donor-reactive T cells for frequency and reactivity showed that the donor-reactive T cells were partially deleted and the rest were rendered unresponsive. CP-induced tolerance may be applied for the induction of antigen-specific tolerance to allogeneic heart transplantation.

    Original languageEnglish
    Pages (from-to)501-507
    Number of pages7
    JournalCellular Immunology
    Volume155
    Issue number2
    DOIs
    Publication statusPublished - Jan 1 1994

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    All Science Journal Classification (ASJC) codes

    • Immunology

    Cite this

    Matsuura, A., Katsuno, M., Suzuki, Y., Ito, T., Yasuura, K., Abe, T., & Yoshikai, Y. (1994). Cyclophosphamide-induced tolerance in fully allogeneic heart transplantation in mice. Cellular Immunology, 155(2), 501-507. https://doi.org/10.1006/cimm.1994.1142