Cytochrome b558, a component of the phagocyte NADPH oxidase, is a flavoprotein

Hideki Sumimoto, Norihiro Sakamoto, Masahiko Nozaki, Yoshiyuki Sakaki, Koichiro Takeshige, Shigeki Minakami

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Cytochrome b558 is the only membrane component of the phagocyte O2- NADPH oxidase. The O2- production by the oxidase reconstituted in vitro with the crude membrane fraction is enhanced several-fold by addition of FAD, whereas that with the partially purified cytochrome is completely dependent on exogenous FAD, suggesting that FAD acts through the membrane component, cytochrome b558. The alignments of the amino acid sequence of the large subunit of the cytochrome (gp91-phox) with those of previously characterized flavoproteins reveal that the middle and C-terminal portions of gp91-phox are likely to be FAD- and NADPHbinding domains, respectively. Cytochrome b558, thus, appears to be a flavoprotein with an NADPH-binding site, of the NADPH oxidase.

Original languageEnglish
Pages (from-to)1368-1375
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume186
Issue number3
DOIs
Publication statusPublished - Aug 14 1992

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Flavoproteins
Flavin-Adenine Dinucleotide
NADPH Oxidase
Phagocytes
Cytochromes
Membranes
NADP
Amino Acid Sequence
Oxidoreductases
Binding Sites
Amino Acids
cytochrome b558
4-ethoxymethylene-2-phenyl-2-oxazoline-5-one

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cytochrome b558, a component of the phagocyte NADPH oxidase, is a flavoprotein. / Sumimoto, Hideki; Sakamoto, Norihiro; Nozaki, Masahiko; Sakaki, Yoshiyuki; Takeshige, Koichiro; Minakami, Shigeki.

In: Biochemical and Biophysical Research Communications, Vol. 186, No. 3, 14.08.1992, p. 1368-1375.

Research output: Contribution to journalArticle

Sumimoto, Hideki ; Sakamoto, Norihiro ; Nozaki, Masahiko ; Sakaki, Yoshiyuki ; Takeshige, Koichiro ; Minakami, Shigeki. / Cytochrome b558, a component of the phagocyte NADPH oxidase, is a flavoprotein. In: Biochemical and Biophysical Research Communications. 1992 ; Vol. 186, No. 3. pp. 1368-1375.
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AU - Takeshige, Koichiro

AU - Minakami, Shigeki

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