Cytochrome c peroxidase from Phanerochaete chrysosporium

Daisuke Nonaka, Hiroyuki Wariishi

Research output: Contribution to journalArticle

Abstract

Cytochrome c peroxidase from the white-rot basidiomycete Phanerochate chrysosporium (PcCcP) was investigated. A phylogenic analysis of PcCcP amino acid sequence showed that PcCcP was closely related to cytochrome c peroxidase from Saccharomyces cerevisiae (yeastCcP) and pea cytosolic ascorbate peroxidase (APX). Recombinant PcCcP was obtained by expression in Eshcherichia coil and a heme incorporation into the apoenzymes. Spectral charactersitics indicated that the heme iron of PcCcP was mainly 5-coordinated high spin species. The absorption spectrum of PcCcP compound I and rapid-scan spectra of compound I formation strongly suggested that PcCcP compound I was ferryoxy heme iron and protein cation radical, as observed in yeastCcP. Although several typical peroxidase substrates, small organic or inorganic compounds, were not oxidized by PcCcP, ferrocytochrome c was effectively oxidized. Both PcCcP and yeastCcP shared catalytic features. A homology modeling of PcCcP and cytochrome c from P. chrysosporium (PcCc) strongly suggested the interaction between PcCcP and PcCc.

Original languageEnglish
Pages (from-to)151-164
Number of pages14
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume50
Issue number1
Publication statusPublished - Feb 2005

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cytochrome-c peroxidase
Cytochrome-c Peroxidase
Phanerochaete
Phanerochaete chrysosporium
heme iron
cytochrome c
Heme
Cytochromes c
Chrysosporium
inorganic compounds
apoproteins
heme
Iron
ascorbate peroxidase
Basidiomycota
organic compounds
Ascorbate Peroxidases
Apoenzymes
Saccharomyces cerevisiae
peas

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Cytochrome c peroxidase from Phanerochaete chrysosporium. / Nonaka, Daisuke; Wariishi, Hiroyuki.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 50, No. 1, 02.2005, p. 151-164.

Research output: Contribution to journalArticle

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