Cytochrome c3 Modified Artificial Liposome. Structure, Electron Transport, and pH Gradient Generation

Iwao Tabushi, Takako Nishiya, Masatsugu Shimomuraf, Toyoki Kunitake, Hiroo Inokuchi, Tatsuhiko Yagi

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Detailed studies on the structure, electron transport rates, and proton transport rates coupled to electron transport were carried out for artificial lecithin liposomes functionalized with cyt c3 or cyt c. Artificial liposomes modified with cyt c3 were found to be single-walled, normal liposomes of ca. 250-Å diameter based on electron micrographs. The electron transport rate across the membrane was very much accelerated in the presence of cyt c3, and the electron influx was coupled with rapid proton transport from the outside to the inside of the artificial liposome, generating a large pH gradient across the membrane. The maximum pH gradient is determined by the accelerated proton influx and slower passive proton efflux. The permeability coefficients of passive proton flow, PH+, and of accelerated coupled proton flow, PH+, were estimated. The observed permeability for the coupled transport is by a factor of l01-l02 larger than the usual passive proton permeability.

Original languageEnglish
Pages (from-to)219-226
Number of pages8
JournalJournal of the American Chemical Society
Volume106
Issue number1
DOIs
Publication statusPublished - Jan 1 1984

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Proton-Motive Force
Liposomes
Electron Transport
Protons
Proteins
Permeability
Electrons
Membranes
Lecithin
Lecithins
Hydraulic conductivity
cytochrome c(3)

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Cytochrome c3 Modified Artificial Liposome. Structure, Electron Transport, and pH Gradient Generation. / Tabushi, Iwao; Nishiya, Takako; Shimomuraf, Masatsugu; Kunitake, Toyoki; Inokuchi, Hiroo; Yagi, Tatsuhiko.

In: Journal of the American Chemical Society, Vol. 106, No. 1, 01.01.1984, p. 219-226.

Research output: Contribution to journalArticle

Tabushi, Iwao ; Nishiya, Takako ; Shimomuraf, Masatsugu ; Kunitake, Toyoki ; Inokuchi, Hiroo ; Yagi, Tatsuhiko. / Cytochrome c3 Modified Artificial Liposome. Structure, Electron Transport, and pH Gradient Generation. In: Journal of the American Chemical Society. 1984 ; Vol. 106, No. 1. pp. 219-226.
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