Cytochrome P450 monooxygenases involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium

Nomathemba Loice Chigu, Sinji Hirosue, Chie Nakamura, Hiroshi Teramoto, Hirofumi Ichinose, Hiroyuki Wariishi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Cytochrome P450 monooxygenases (P450s) involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium were identified by comprehensive screening of both catalytic potentials and transcriptomic profiling. Functional screening of P. chrysosporium P450s (PcCYPs) revealed that 14 PcCYP species catalyze stepwise conversion of anthracene to anthraquinone via intermediate formation of anthrone. Moreover, transcriptomic profiling explored using a complementary DNA microarray system demonstrated that 12 PcCYPs are up-regulated in response to exogenous addition of anthracene. Among the up-regulated PcCYPs, five species showed catalytic activity against anthracene. Based upon both catalytic and transcriptional properties, these five species are most likely to play major roles in anthracene metabolic processes in vivo. Thus, the combination of functional screening and a microarray system may provide a novel strategy for obtaining a thorough understanding of the catalytic functions and biological impacts of PcCYPs.

Original languageEnglish
Pages (from-to)1907-1916
Number of pages10
JournalApplied Microbiology and Biotechnology
Volume87
Issue number5
DOIs
Publication statusPublished - Aug 1 2010

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Phanerochaete
Basidiomycota
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Anthraquinones
Oligonucleotide Array Sequence Analysis
Complementary DNA
anthracene

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Cytochrome P450 monooxygenases involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium. / Chigu, Nomathemba Loice; Hirosue, Sinji; Nakamura, Chie; Teramoto, Hiroshi; Ichinose, Hirofumi; Wariishi, Hiroyuki.

In: Applied Microbiology and Biotechnology, Vol. 87, No. 5, 01.08.2010, p. 1907-1916.

Research output: Contribution to journalArticle

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