Cytokeratins 18 and 8 are poor prognostic markers in patients with squamous cell carcinoma of the oesophagus

T. Makino, M. Yamasaki, A. Takeno, M. Shirakawa, H. Miyata, S. Takiguchi, K. Nakajima, Y. Fujiwara, T. Nishida, N. Matsuura, Masaki Mori, Y. Doki

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Abstract

Background:Cytokeratins (CKs) are structural marker proteins specific for epithelial cells. However, recent studies indicate their involvement in cancer progression.Methods:We evaluated CK18 and its filament partner, CK8 expression, by immunohistochemistry in 210 resected specimens from patients with oesophageal squamous cell carcinoma (OSCC). We also analysed the relationship between their expression and various clinicopathological parameters including prognosis.Results:Neither CK18 nor CK8 was expressed in non-cancerous squamous epithelium whereas proper oesophageal glands expressed both CKs. Ninety (42.9%) tumours were CK18 positive and 85 (40.5%) CK8 positive, and the concordance rate for immunohistochemical classification for CK18 and CK8 was 82.4%. CK18 expression correlated with poorly differentiated tumours, use of neo-adjuvant chemotherapy, and advanced stage. Prognosis of patients with CK18-positive tumours was poorer than that of patients with negative OSCC (P0.001). A similar trend was noted for CK8 expression. Multivariate analysis identified pT (P0.020), pN number (P0.001), and CK18 expression (P0.004) as independent prognostic factors. CK18 expression in 83 pretreatment biopsy specimens was detected in 47 cases (56.6%) and also correlated with prognosis (P0.045).Conclusion:CK18/CK8 expression correlated with progression of OSCC. The significant correlation with prognosis and stable expression in biopsy specimen suggest usefulness of CK18 in selection of treatment strategies for OSCC.

Original languageEnglish
Pages (from-to)1298-1306
Number of pages9
JournalBritish journal of cancer
Volume101
Issue number8
DOIs
Publication statusPublished - Oct 20 2009

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Keratin-8
Keratin-18
Esophagus
Squamous Cell Carcinoma
Keratins
Neoplasms
Biopsy
Adjuvant Chemotherapy
Multivariate Analysis
Epithelium
Epithelial Cells
Immunohistochemistry
Esophageal Squamous Cell Carcinoma
Proteins

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Makino, T., Yamasaki, M., Takeno, A., Shirakawa, M., Miyata, H., Takiguchi, S., ... Doki, Y. (2009). Cytokeratins 18 and 8 are poor prognostic markers in patients with squamous cell carcinoma of the oesophagus. British journal of cancer, 101(8), 1298-1306. https://doi.org/10.1038/sj.bjc.6605313

Cytokeratins 18 and 8 are poor prognostic markers in patients with squamous cell carcinoma of the oesophagus. / Makino, T.; Yamasaki, M.; Takeno, A.; Shirakawa, M.; Miyata, H.; Takiguchi, S.; Nakajima, K.; Fujiwara, Y.; Nishida, T.; Matsuura, N.; Mori, Masaki; Doki, Y.

In: British journal of cancer, Vol. 101, No. 8, 20.10.2009, p. 1298-1306.

Research output: Contribution to journalArticle

Makino, T, Yamasaki, M, Takeno, A, Shirakawa, M, Miyata, H, Takiguchi, S, Nakajima, K, Fujiwara, Y, Nishida, T, Matsuura, N, Mori, M & Doki, Y 2009, 'Cytokeratins 18 and 8 are poor prognostic markers in patients with squamous cell carcinoma of the oesophagus', British journal of cancer, vol. 101, no. 8, pp. 1298-1306. https://doi.org/10.1038/sj.bjc.6605313
Makino, T. ; Yamasaki, M. ; Takeno, A. ; Shirakawa, M. ; Miyata, H. ; Takiguchi, S. ; Nakajima, K. ; Fujiwara, Y. ; Nishida, T. ; Matsuura, N. ; Mori, Masaki ; Doki, Y. / Cytokeratins 18 and 8 are poor prognostic markers in patients with squamous cell carcinoma of the oesophagus. In: British journal of cancer. 2009 ; Vol. 101, No. 8. pp. 1298-1306.
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abstract = "Background:Cytokeratins (CKs) are structural marker proteins specific for epithelial cells. However, recent studies indicate their involvement in cancer progression.Methods:We evaluated CK18 and its filament partner, CK8 expression, by immunohistochemistry in 210 resected specimens from patients with oesophageal squamous cell carcinoma (OSCC). We also analysed the relationship between their expression and various clinicopathological parameters including prognosis.Results:Neither CK18 nor CK8 was expressed in non-cancerous squamous epithelium whereas proper oesophageal glands expressed both CKs. Ninety (42.9{\%}) tumours were CK18 positive and 85 (40.5{\%}) CK8 positive, and the concordance rate for immunohistochemical classification for CK18 and CK8 was 82.4{\%}. CK18 expression correlated with poorly differentiated tumours, use of neo-adjuvant chemotherapy, and advanced stage. Prognosis of patients with CK18-positive tumours was poorer than that of patients with negative OSCC (P0.001). A similar trend was noted for CK8 expression. Multivariate analysis identified pT (P0.020), pN number (P0.001), and CK18 expression (P0.004) as independent prognostic factors. CK18 expression in 83 pretreatment biopsy specimens was detected in 47 cases (56.6{\%}) and also correlated with prognosis (P0.045).Conclusion:CK18/CK8 expression correlated with progression of OSCC. The significant correlation with prognosis and stable expression in biopsy specimen suggest usefulness of CK18 in selection of treatment strategies for OSCC.",
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AU - Makino, T.

AU - Yamasaki, M.

AU - Takeno, A.

AU - Shirakawa, M.

AU - Miyata, H.

AU - Takiguchi, S.

AU - Nakajima, K.

AU - Fujiwara, Y.

AU - Nishida, T.

AU - Matsuura, N.

AU - Mori, Masaki

AU - Doki, Y.

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