Cytokine-independent Jak3 Activation upon T Cell Receptor (TCR) Stimulation through Direct Association of Jak3 and the TCR Complex

Kazuhiro Tomita, Kaoru Saijo, Sho Yamasaki, Tomohiko Iida, Fubito Nakatsu, Hisashi Arase, Hiroshi Ohno, Takuji Shirasawa, Takayuki Kuriyama, John J. O'Shea, Takashi Saito

    Research output: Contribution to journalArticle

    29 Citations (Scopus)

    Abstract

    Jak3 is responsible for growth signals by various cytokines such as interleukin (IL)-2, IL-4, and IL-7 through association with the common γ chain (γc) in lymphocytes. We found that T cells from Jak3-deficient mice exhibit impairment of not only cytokine signaling but also early activation signals and that Jak3 is phosphorylated upon T cell receptor (TCR) stimulation. TCR-mediated phosphorylation of Jak3 is independent of IL-2 receptor/γc but is dependent on Lck and ZAP-70. Jak3 was found to be assembled with the TCR complex, particularly through direct association with CD3ζ via its JH4 region, which is a different region from that for γc association. These results suggest that Jak3 plays a role not only in cell growth but also in T cell activation and represents cross-talk of a signaling molecule between TCR and growth signals.

    Original languageEnglish
    Pages (from-to)25378-25385
    Number of pages8
    JournalJournal of Biological Chemistry
    Volume276
    Issue number27
    DOIs
    Publication statusPublished - Jul 6 2001

    Fingerprint

    T-Cell Antigen Receptor
    Chemical activation
    Cytokines
    T-cells
    Association reactions
    Growth
    T-Lymphocytes
    Interleukin-7
    Phosphorylation
    Lymphocytes
    Interleukin-2 Receptors
    Cell growth
    Interleukin-4
    Interleukin-2
    Molecules

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Cite this

    Cytokine-independent Jak3 Activation upon T Cell Receptor (TCR) Stimulation through Direct Association of Jak3 and the TCR Complex. / Tomita, Kazuhiro; Saijo, Kaoru; Yamasaki, Sho; Iida, Tomohiko; Nakatsu, Fubito; Arase, Hisashi; Ohno, Hiroshi; Shirasawa, Takuji; Kuriyama, Takayuki; O'Shea, John J.; Saito, Takashi.

    In: Journal of Biological Chemistry, Vol. 276, No. 27, 06.07.2001, p. 25378-25385.

    Research output: Contribution to journalArticle

    Tomita, K, Saijo, K, Yamasaki, S, Iida, T, Nakatsu, F, Arase, H, Ohno, H, Shirasawa, T, Kuriyama, T, O'Shea, JJ & Saito, T 2001, 'Cytokine-independent Jak3 Activation upon T Cell Receptor (TCR) Stimulation through Direct Association of Jak3 and the TCR Complex', Journal of Biological Chemistry, vol. 276, no. 27, pp. 25378-25385. https://doi.org/10.1074/jbc.M011363200
    Tomita, Kazuhiro ; Saijo, Kaoru ; Yamasaki, Sho ; Iida, Tomohiko ; Nakatsu, Fubito ; Arase, Hisashi ; Ohno, Hiroshi ; Shirasawa, Takuji ; Kuriyama, Takayuki ; O'Shea, John J. ; Saito, Takashi. / Cytokine-independent Jak3 Activation upon T Cell Receptor (TCR) Stimulation through Direct Association of Jak3 and the TCR Complex. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 27. pp. 25378-25385.
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    abstract = "Jak3 is responsible for growth signals by various cytokines such as interleukin (IL)-2, IL-4, and IL-7 through association with the common γ chain (γc) in lymphocytes. We found that T cells from Jak3-deficient mice exhibit impairment of not only cytokine signaling but also early activation signals and that Jak3 is phosphorylated upon T cell receptor (TCR) stimulation. TCR-mediated phosphorylation of Jak3 is independent of IL-2 receptor/γc but is dependent on Lck and ZAP-70. Jak3 was found to be assembled with the TCR complex, particularly through direct association with CD3ζ via its JH4 region, which is a different region from that for γc association. These results suggest that Jak3 plays a role not only in cell growth but also in T cell activation and represents cross-talk of a signaling molecule between TCR and growth signals.",
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    AU - Saijo, Kaoru

    AU - Yamasaki, Sho

    AU - Iida, Tomohiko

    AU - Nakatsu, Fubito

    AU - Arase, Hisashi

    AU - Ohno, Hiroshi

    AU - Shirasawa, Takuji

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    AB - Jak3 is responsible for growth signals by various cytokines such as interleukin (IL)-2, IL-4, and IL-7 through association with the common γ chain (γc) in lymphocytes. We found that T cells from Jak3-deficient mice exhibit impairment of not only cytokine signaling but also early activation signals and that Jak3 is phosphorylated upon T cell receptor (TCR) stimulation. TCR-mediated phosphorylation of Jak3 is independent of IL-2 receptor/γc but is dependent on Lck and ZAP-70. Jak3 was found to be assembled with the TCR complex, particularly through direct association with CD3ζ via its JH4 region, which is a different region from that for γc association. These results suggest that Jak3 plays a role not only in cell growth but also in T cell activation and represents cross-talk of a signaling molecule between TCR and growth signals.

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