Cytokine messenger RNA expression in the labial salivary glands of patients with Sjogren's syndrome

Yukiko Ohyama, Seiji Nakamura, Goro Matsuzaki, Masanori Shinohara, Akiko Hiroki, Takao Fujimura, Akira Yamada, Kyogo Itoh, Kikuo Nomoto

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

Objective. To characterize the initiation and progress of localized autoimmune damage in Sjogren's syndrome (SS), an autoimmune disease that is also considered to be a lymphoaggressive disorder, by examining the pattern of cytokine production at the site of autoimmune damage. Methods. Using a polymerase chain reaction-based method, cytokine messenger RNA (mRNA) expression in the labial salivary glands of 15 patients with SS was investigated. In addition, the infiltrating lymphocytes in the labial salivary glands were examined immunohistochemically. Results. Messenger RNAs of Th1 cytokines, such as interleukin-2 (IL-2) and interferon-γ, were consistently detected in all patients, while Th2 cytokine mRNAs, such as IL- 4 and IL-5, were detected in some cases, in association with strong B cell accumulation in the labial salivary glands. Other cytokine mRNAs produced by a variety of cell types, including IL-10, IL-6, and transforming growth factor β (TGFβ), were also consistently detected in all patients, while IL- 12 mRNA was detected in some of the patients. Conclusion. These results suggest that Th1 cytokines, as well as IL-10, IL-6, and TGFβ, are essential in the induction and/or maintenance of SS, while Th2 cytokines are involved in the progression of the disease process, especially local B cell activation.

Original languageEnglish
Pages (from-to)1376-1384
Number of pages9
JournalArthritis and rheumatism
Volume39
Issue number8
DOIs
Publication statusPublished - Jan 1 1996

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Sjogren's Syndrome
Lip
Salivary Glands
Cytokines
Messenger RNA
Transforming Growth Factors
Interleukin-10
Interleukin-6
B-Lymphocytes
Interleukin-5
Interleukin-12
Interleukin-4
Interferons
Autoimmune Diseases
Interleukin-2
Disease Progression
Maintenance
Lymphocytes
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

Cite this

Cytokine messenger RNA expression in the labial salivary glands of patients with Sjogren's syndrome. / Ohyama, Yukiko; Nakamura, Seiji; Matsuzaki, Goro; Shinohara, Masanori; Hiroki, Akiko; Fujimura, Takao; Yamada, Akira; Itoh, Kyogo; Nomoto, Kikuo.

In: Arthritis and rheumatism, Vol. 39, No. 8, 01.01.1996, p. 1376-1384.

Research output: Contribution to journalArticle

Ohyama, Y, Nakamura, S, Matsuzaki, G, Shinohara, M, Hiroki, A, Fujimura, T, Yamada, A, Itoh, K & Nomoto, K 1996, 'Cytokine messenger RNA expression in the labial salivary glands of patients with Sjogren's syndrome', Arthritis and rheumatism, vol. 39, no. 8, pp. 1376-1384. https://doi.org/10.1002/art.1780390816
Ohyama, Yukiko ; Nakamura, Seiji ; Matsuzaki, Goro ; Shinohara, Masanori ; Hiroki, Akiko ; Fujimura, Takao ; Yamada, Akira ; Itoh, Kyogo ; Nomoto, Kikuo. / Cytokine messenger RNA expression in the labial salivary glands of patients with Sjogren's syndrome. In: Arthritis and rheumatism. 1996 ; Vol. 39, No. 8. pp. 1376-1384.
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