Cytoplasmic expression and extracellular deposition of an antiangiogenic factor, pigment epithelium-derived factor, in human atherosclerotic plaques

Hiromitsu Baba, Yoshikazu Yonemitsu, Toshiaki Nakano, Mitsuho Onimaru, Masanori Miyazaki, Yasuhiro Ikeda, Shinji Sumiyoshi, Yasuji Ueda, Mamoru Hasegawa, Ichiro Yoshino, Yoshihiko Maehara, Katsuo Sueishi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Objective - To assess the expression and distribution of a neurotrophic/antiangiogenic factor, pigment epithelium-derived factor (PEDF), related to angiogenesis that is a possibly key event during atherogenesis in human atherosclerotic plaques. Methods and Results - Twenty fresh aortic samples were used for reverse-transcription polymerase chain reaction (RT-PCR), Western blot, and immunohistochemistry (IHC). In addition, 80 stocked paraffin blocks of coronary arteries from 40 autopsy cases were also used. IHC revealed divergent staining patterns for PEDF in both the aortas and the coronary arteries tested, ie, "cytoplasmic staining" or "extracellular deposition," were observed, respectively. In the areas showing cytoplasmic staining, double PEDF was expressed in a majority of the foamy macrophages and in some smooth muscle cells, and the PEDF-positive cell frequency was positively correlated with that of microvessels in a cell-rich area in the coronary arteries (P<0.0001). Inversely, extracellular deposition of PEDF was seen in acellular areas and was negatively correlated with the number of microvessels (P=0.0003). Conclusions - These results suggest that PEDF may function as an antiangiogenic factor when it is deposited onto the extracellular matrix. Thus, PEDF may play a significant role in determining the balance of angiogenesis/antiangiogenesis during atherogenesis.

Original languageEnglish
Pages (from-to)1938-1944
Number of pages7
JournalArteriosclerosis, thrombosis, and vascular biology
Volume25
Issue number9
DOIs
Publication statusPublished - Sep 1 2005

Fingerprint

Atherosclerotic Plaques
Coronary Vessels
Staining and Labeling
Microvessels
Atherosclerosis
Immunohistochemistry
Nerve Growth Factors
pigment epithelium-derived factor
Paraffin
Reverse Transcription
Smooth Muscle Myocytes
Extracellular Matrix
Aorta
Autopsy
Western Blotting
Macrophages
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Cytoplasmic expression and extracellular deposition of an antiangiogenic factor, pigment epithelium-derived factor, in human atherosclerotic plaques. / Baba, Hiromitsu; Yonemitsu, Yoshikazu; Nakano, Toshiaki; Onimaru, Mitsuho; Miyazaki, Masanori; Ikeda, Yasuhiro; Sumiyoshi, Shinji; Ueda, Yasuji; Hasegawa, Mamoru; Yoshino, Ichiro; Maehara, Yoshihiko; Sueishi, Katsuo.

In: Arteriosclerosis, thrombosis, and vascular biology, Vol. 25, No. 9, 01.09.2005, p. 1938-1944.

Research output: Contribution to journalArticle

Baba, Hiromitsu ; Yonemitsu, Yoshikazu ; Nakano, Toshiaki ; Onimaru, Mitsuho ; Miyazaki, Masanori ; Ikeda, Yasuhiro ; Sumiyoshi, Shinji ; Ueda, Yasuji ; Hasegawa, Mamoru ; Yoshino, Ichiro ; Maehara, Yoshihiko ; Sueishi, Katsuo. / Cytoplasmic expression and extracellular deposition of an antiangiogenic factor, pigment epithelium-derived factor, in human atherosclerotic plaques. In: Arteriosclerosis, thrombosis, and vascular biology. 2005 ; Vol. 25, No. 9. pp. 1938-1944.
@article{7b2472870eb041488d3fa2e077417300,
title = "Cytoplasmic expression and extracellular deposition of an antiangiogenic factor, pigment epithelium-derived factor, in human atherosclerotic plaques",
abstract = "Objective - To assess the expression and distribution of a neurotrophic/antiangiogenic factor, pigment epithelium-derived factor (PEDF), related to angiogenesis that is a possibly key event during atherogenesis in human atherosclerotic plaques. Methods and Results - Twenty fresh aortic samples were used for reverse-transcription polymerase chain reaction (RT-PCR), Western blot, and immunohistochemistry (IHC). In addition, 80 stocked paraffin blocks of coronary arteries from 40 autopsy cases were also used. IHC revealed divergent staining patterns for PEDF in both the aortas and the coronary arteries tested, ie, {"}cytoplasmic staining{"} or {"}extracellular deposition,{"} were observed, respectively. In the areas showing cytoplasmic staining, double PEDF was expressed in a majority of the foamy macrophages and in some smooth muscle cells, and the PEDF-positive cell frequency was positively correlated with that of microvessels in a cell-rich area in the coronary arteries (P<0.0001). Inversely, extracellular deposition of PEDF was seen in acellular areas and was negatively correlated with the number of microvessels (P=0.0003). Conclusions - These results suggest that PEDF may function as an antiangiogenic factor when it is deposited onto the extracellular matrix. Thus, PEDF may play a significant role in determining the balance of angiogenesis/antiangiogenesis during atherogenesis.",
author = "Hiromitsu Baba and Yoshikazu Yonemitsu and Toshiaki Nakano and Mitsuho Onimaru and Masanori Miyazaki and Yasuhiro Ikeda and Shinji Sumiyoshi and Yasuji Ueda and Mamoru Hasegawa and Ichiro Yoshino and Yoshihiko Maehara and Katsuo Sueishi",
year = "2005",
month = "9",
day = "1",
doi = "10.1161/01.ATV.0000175759.78338.1e",
language = "English",
volume = "25",
pages = "1938--1944",
journal = "Arteriosclerosis, Thrombosis, and Vascular Biology",
issn = "1079-5642",
publisher = "Lippincott Williams and Wilkins",
number = "9",

}

TY - JOUR

T1 - Cytoplasmic expression and extracellular deposition of an antiangiogenic factor, pigment epithelium-derived factor, in human atherosclerotic plaques

AU - Baba, Hiromitsu

AU - Yonemitsu, Yoshikazu

AU - Nakano, Toshiaki

AU - Onimaru, Mitsuho

AU - Miyazaki, Masanori

AU - Ikeda, Yasuhiro

AU - Sumiyoshi, Shinji

AU - Ueda, Yasuji

AU - Hasegawa, Mamoru

AU - Yoshino, Ichiro

AU - Maehara, Yoshihiko

AU - Sueishi, Katsuo

PY - 2005/9/1

Y1 - 2005/9/1

N2 - Objective - To assess the expression and distribution of a neurotrophic/antiangiogenic factor, pigment epithelium-derived factor (PEDF), related to angiogenesis that is a possibly key event during atherogenesis in human atherosclerotic plaques. Methods and Results - Twenty fresh aortic samples were used for reverse-transcription polymerase chain reaction (RT-PCR), Western blot, and immunohistochemistry (IHC). In addition, 80 stocked paraffin blocks of coronary arteries from 40 autopsy cases were also used. IHC revealed divergent staining patterns for PEDF in both the aortas and the coronary arteries tested, ie, "cytoplasmic staining" or "extracellular deposition," were observed, respectively. In the areas showing cytoplasmic staining, double PEDF was expressed in a majority of the foamy macrophages and in some smooth muscle cells, and the PEDF-positive cell frequency was positively correlated with that of microvessels in a cell-rich area in the coronary arteries (P<0.0001). Inversely, extracellular deposition of PEDF was seen in acellular areas and was negatively correlated with the number of microvessels (P=0.0003). Conclusions - These results suggest that PEDF may function as an antiangiogenic factor when it is deposited onto the extracellular matrix. Thus, PEDF may play a significant role in determining the balance of angiogenesis/antiangiogenesis during atherogenesis.

AB - Objective - To assess the expression and distribution of a neurotrophic/antiangiogenic factor, pigment epithelium-derived factor (PEDF), related to angiogenesis that is a possibly key event during atherogenesis in human atherosclerotic plaques. Methods and Results - Twenty fresh aortic samples were used for reverse-transcription polymerase chain reaction (RT-PCR), Western blot, and immunohistochemistry (IHC). In addition, 80 stocked paraffin blocks of coronary arteries from 40 autopsy cases were also used. IHC revealed divergent staining patterns for PEDF in both the aortas and the coronary arteries tested, ie, "cytoplasmic staining" or "extracellular deposition," were observed, respectively. In the areas showing cytoplasmic staining, double PEDF was expressed in a majority of the foamy macrophages and in some smooth muscle cells, and the PEDF-positive cell frequency was positively correlated with that of microvessels in a cell-rich area in the coronary arteries (P<0.0001). Inversely, extracellular deposition of PEDF was seen in acellular areas and was negatively correlated with the number of microvessels (P=0.0003). Conclusions - These results suggest that PEDF may function as an antiangiogenic factor when it is deposited onto the extracellular matrix. Thus, PEDF may play a significant role in determining the balance of angiogenesis/antiangiogenesis during atherogenesis.

UR - http://www.scopus.com/inward/record.url?scp=24144474977&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24144474977&partnerID=8YFLogxK

U2 - 10.1161/01.ATV.0000175759.78338.1e

DO - 10.1161/01.ATV.0000175759.78338.1e

M3 - Article

C2 - 15994443

AN - SCOPUS:24144474977

VL - 25

SP - 1938

EP - 1944

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

SN - 1079-5642

IS - 9

ER -