Cytoplasmic expression and extracellular deposition of an antiangiogenic factor, pigment epithelium-derived factor, in human atherosclerotic plaques

Hiromitsu Baba, Yoshikazu Yonemitsu, Toshiaki Nakano, Mitsuho Onimaru, Masanori Miyazaki, Yasuhiro Ikeda, Shinji Sumiyoshi, Yasuji Ueda, Mamoru Hasegawa, Ichiro Yoshino, Yoshihiko Maehara, Katsuo Sueishi

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Objective - To assess the expression and distribution of a neurotrophic/antiangiogenic factor, pigment epithelium-derived factor (PEDF), related to angiogenesis that is a possibly key event during atherogenesis in human atherosclerotic plaques. Methods and Results - Twenty fresh aortic samples were used for reverse-transcription polymerase chain reaction (RT-PCR), Western blot, and immunohistochemistry (IHC). In addition, 80 stocked paraffin blocks of coronary arteries from 40 autopsy cases were also used. IHC revealed divergent staining patterns for PEDF in both the aortas and the coronary arteries tested, ie, "cytoplasmic staining" or "extracellular deposition," were observed, respectively. In the areas showing cytoplasmic staining, double PEDF was expressed in a majority of the foamy macrophages and in some smooth muscle cells, and the PEDF-positive cell frequency was positively correlated with that of microvessels in a cell-rich area in the coronary arteries (P<0.0001). Inversely, extracellular deposition of PEDF was seen in acellular areas and was negatively correlated with the number of microvessels (P=0.0003). Conclusions - These results suggest that PEDF may function as an antiangiogenic factor when it is deposited onto the extracellular matrix. Thus, PEDF may play a significant role in determining the balance of angiogenesis/antiangiogenesis during atherogenesis.

Original languageEnglish
Pages (from-to)1938-1944
Number of pages7
JournalArteriosclerosis, thrombosis, and vascular biology
Issue number9
Publication statusPublished - Sep 1 2005


All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

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