Cytoplasmic maturation for activation of pig follicular oocytes cultured and arrested at metaphase I

K. Kikuchi, T. Nagai, J. Ding, N. Yamauchi, J. Noguchi, Y. Izaike

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

A large population (62-90%) of pig follicular oocytes can mature to metaphase II after culture for 48 h. However, a proportion (6-22%) remain in an immature stage at metaphase I (metaphase I-arrested). The main objective of this study was to determine whether the cytoplasm of metaphase I-arrested pig oocytes is capable of being activated by sperm penetration or parthenogenetic stimulation. After culture for 48 h, oocytes without a polar body (73% were shown to be at metaphase I after staining) and those with a polar body (94% were at metaphase II) were fertilized in vitro. A total of 69% and 62% of the oocytes were activated to form a female pronucleus, respectively, and the rate of polar body extrusion induced by fertilization in the activated oocytes was 90% (the first polar body) and 95% (the second polar body), respectively. When oocytes without and with a polar body were stimulated with an electric pulse, 53% and 81% of the oocytes were activated, respectively. The rate of polar body extrusion in the activated oocytes was 73% (the first polar body) and 79% (the second polar body), respectively. In contrast, young metaphase I oocytes cultured for 24 h had low (6%) or zero activation rate after in vitro fertilization or electric pulse stimulation. However, about one-third of the young metaphase I oocytes penetrated by spermatozoa after in vitro fertilization responded to electric pulse 12 h after insemination, and almost all (93%) were activated when they were stimulated 24 h after insemination. Patterns of polypeptide synthesis and histone H1 kinase activity were similar in metaphase I-arrested and metaphase II oocytes, and were characterized by increase in a 25 kDa polypeptide and by decrease in kinase activity. Although the first step of meiotic division is impaired, these results indicate that metaphase I-arrested oocytes are mature cytoplasmically.

Original languageEnglish
Pages (from-to)143-156
Number of pages14
JournalJournal of Reproduction and Fertility
Volume116
Issue number1
DOIs
Publication statusPublished - May 1999
Externally publishedYes

Fingerprint

Metaphase
Polar Bodies
Oocytes
Swine
Insemination
Fertilization in Vitro
Sperm-Ovum Interactions
Peptides
Fertilization
Electric Stimulation
Spermatozoa
Cytoplasm
Phosphotransferases
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Physiology
  • Embryology
  • Molecular Biology
  • Obstetrics and Gynaecology
  • Developmental Biology

Cite this

Cytoplasmic maturation for activation of pig follicular oocytes cultured and arrested at metaphase I. / Kikuchi, K.; Nagai, T.; Ding, J.; Yamauchi, N.; Noguchi, J.; Izaike, Y.

In: Journal of Reproduction and Fertility, Vol. 116, No. 1, 05.1999, p. 143-156.

Research output: Contribution to journalArticle

Kikuchi, K. ; Nagai, T. ; Ding, J. ; Yamauchi, N. ; Noguchi, J. ; Izaike, Y. / Cytoplasmic maturation for activation of pig follicular oocytes cultured and arrested at metaphase I. In: Journal of Reproduction and Fertility. 1999 ; Vol. 116, No. 1. pp. 143-156.
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abstract = "A large population (62-90{\%}) of pig follicular oocytes can mature to metaphase II after culture for 48 h. However, a proportion (6-22{\%}) remain in an immature stage at metaphase I (metaphase I-arrested). The main objective of this study was to determine whether the cytoplasm of metaphase I-arrested pig oocytes is capable of being activated by sperm penetration or parthenogenetic stimulation. After culture for 48 h, oocytes without a polar body (73{\%} were shown to be at metaphase I after staining) and those with a polar body (94{\%} were at metaphase II) were fertilized in vitro. A total of 69{\%} and 62{\%} of the oocytes were activated to form a female pronucleus, respectively, and the rate of polar body extrusion induced by fertilization in the activated oocytes was 90{\%} (the first polar body) and 95{\%} (the second polar body), respectively. When oocytes without and with a polar body were stimulated with an electric pulse, 53{\%} and 81{\%} of the oocytes were activated, respectively. The rate of polar body extrusion in the activated oocytes was 73{\%} (the first polar body) and 79{\%} (the second polar body), respectively. In contrast, young metaphase I oocytes cultured for 24 h had low (6{\%}) or zero activation rate after in vitro fertilization or electric pulse stimulation. However, about one-third of the young metaphase I oocytes penetrated by spermatozoa after in vitro fertilization responded to electric pulse 12 h after insemination, and almost all (93{\%}) were activated when they were stimulated 24 h after insemination. Patterns of polypeptide synthesis and histone H1 kinase activity were similar in metaphase I-arrested and metaphase II oocytes, and were characterized by increase in a 25 kDa polypeptide and by decrease in kinase activity. Although the first step of meiotic division is impaired, these results indicate that metaphase I-arrested oocytes are mature cytoplasmically.",
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