Cytotoxic drug and cytotoxic drug/G-CSF mobilization of peripheral blood stem cells and their use for autografting

T. Teshima, M. Harada, Y. Takamatsu, K. Makino, S. Taniguchi, S. Inaba, S. Kondo, T. Tanaka, K. Akashi, I. Minamishima, E. Ishii, J. Nishimura, Y. Niho

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Abstract

We analysed 99 courses of leukapheresis after the use of cytotoxic drugs or cytotoxic drugs plus G-CSF (cytotoxic/G-CSF) to mobilize peripheral blood stem cells (PBSC) in 68 patients with hematologic or solid malignancies. Mean yields of granulocyte-macrophage progenitor cells (CFU-GM) with cytotoxic/G-CSF mobilization were significantly higher than those with cytotoxic mobilization (18.6 vs 8.40 X 104/kg). The optimal timing of collection was different between these two mobilizations; the mean number of days to a peak level of circulating CFU-GM after cytotoxic/G-CSF mobilization was less than that after cytotoxic mobilization (24.2 vs 27.7 days). The leukocyte level on the day of peak CFU-GM was significantly higher in cytotoxic/G-CSF mobilization than that in cytotoxic mobilization (mean 12.8 vs 2.7 X 109/I), whereas the platelet level was not different (mean 132 vs 125 x 109/I). Increasing patient age was not a major adverse factor for PBSC collection. Synchronous recovery of both leukocytes and platelets was critical for achieving a high CFU-GM yield in these two mobilizations. Following PBSC autotransplantation, the rate of trilineage hematologic reconstitution showed a significant correlation with the infused dose of CFU-GM, whether they were collected with cytotoxic or cytotoxic/G-CSF mobilization. These results suggest that G-CSF can expand the PBSC pool and that CFU-GM yield after cytotoxic/G-CSF mobilization may predict trilineage hemopoietic reconstitution after ABSCT, as well as cytotoxic mobilization.

Original languageEnglish
Pages (from-to)215-220
Number of pages6
JournalBone Marrow Transplantation
Volume10
Issue number3
Publication statusPublished - Oct 9 1992

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All Science Journal Classification (ASJC) codes

  • Hematology
  • Transplantation

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Teshima, T., Harada, M., Takamatsu, Y., Makino, K., Taniguchi, S., Inaba, S., ... Niho, Y. (1992). Cytotoxic drug and cytotoxic drug/G-CSF mobilization of peripheral blood stem cells and their use for autografting. Bone Marrow Transplantation, 10(3), 215-220.