Cytotoxicity induced by erythrina variegata serine proteinase inhibitors in tumor hematopoietic stem cell lines

Hideki Ohba, Masaaki Nishikawa, Makoto Kimura, Nobuyuki Yamasaki, Sawako Moriwaki, Kyogo Itoh

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Based on the soluble MTT tetrazolium/formazan assay, we evaluated the cytotoxicity of Erythrina variegata proteinase inhibitors in some tumor hematopoietic stem cell lines. Among the proteinase inhibitors, EBI, which belongs to the Bowman-Birk family of inhibitors, was cytotoxic in relatively differentiated cells such as Molt4 and Jurkat derived from acute T lymphoblastic leukemia (T-ALL) cells specifically, but ETIa and ECI, which are classified into Kunitz family inhibitors, did not. It was suggested that the differences in the cytotoxicity might be due to the molecular size of the inhibitors. The succinylation of lysine residue(s) of EBI led to about 50% loss of the trypsin inhibitory activity as compared with the authentic EBI. When Molt4 cells were incubated with this derivative, no significant cytotoxicity was observed. This suggests that the proteinase inhibitory activity might be involved in the cytotoxicity in human tumor cell lines.

Original languageEnglish
Pages (from-to)1166-1170
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number6
DOIs
Publication statusPublished - Jan 1 1998

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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