Cytotoxicity of one-step dentin-bonding agents toward dental pulp and odontoblast-like cells

Y. Yasuda, H. Inuyama, H. Maeda, A. Akamine, J. E. NÖr, T. Saito

Research output: Contribution to journalArticle

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Abstract

The purpose of this study was to compare the cytotoxicity of five one-step dentin-bonding agents on human dental pulp and odontoblast-like cells (MDPC-23). Photopolymerized and unpolymerized samples of these dentin-bonding agents were prepared and incubated with dental pulp or MDPC-23 cells. After 24 or 72 h of incubation, the number of unstained cells with trypan blue was counted. The staining of cells with trypan blue stands for a cytotoxicity. The pulp cell and MDPC-23 cytotoxicity of polymerized sample treatment increased in the order of AQ Bond Plus (AQ)<Clearfil Tri-S Bond (TS)=G-bond (GB)<Absolute (AB)<Adper Prompt (AP) for 24 and 72 h. The pulp cell cytotoxicity of unpolymerized sample treatment for 24 h increased in the order of AQ<GB = AB<TS<AP. The MDPC-23 cytotoxicity of unpolymerized sample treatment for 24 h increased in the order of AQ<GB<TS = AB<AP. Whether polymerized or unpolymerized, AQ was the least cytotoxic agent, while AP was the strongest. All polymerized dentin-bonding agents exhibited lower cytotoxicity by 2-65% than their unpolymerized counterparts. The appearance of the cytotoxicity of dentin-bonding agents was time-dependent, and cell viability was lower at 72 h by 2-46% than at 24 h. The cytotoxicity to MDPC-23 cells was about 5-24% higher than that to pulp cells. These results indicate that one-step dentin-bonding agents differ markedly in their cytotoxicity. Differential cytotoxic effects of one-step dentin-bonding agents should be considered during clinical application of operative restoration.

Original languageEnglish
Pages (from-to)940-946
Number of pages7
JournalJournal of oral rehabilitation
Volume35
Issue number12
DOIs
Publication statusPublished - Dec 1 2008

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Dentin-Bonding Agents
Odontoblasts
Dental Pulp
Trypan Blue
Cytotoxins
Cell Survival
Cell Count
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Cytotoxicity of one-step dentin-bonding agents toward dental pulp and odontoblast-like cells. / Yasuda, Y.; Inuyama, H.; Maeda, H.; Akamine, A.; NÖr, J. E.; Saito, T.

In: Journal of oral rehabilitation, Vol. 35, No. 12, 01.12.2008, p. 940-946.

Research output: Contribution to journalArticle

Yasuda, Y. ; Inuyama, H. ; Maeda, H. ; Akamine, A. ; NÖr, J. E. ; Saito, T. / Cytotoxicity of one-step dentin-bonding agents toward dental pulp and odontoblast-like cells. In: Journal of oral rehabilitation. 2008 ; Vol. 35, No. 12. pp. 940-946.
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