Cytotoxity of the trans10,cis12 isomer of conjugated linoleic acid on rat hepatoma and its modulation by other fatty acids, tocopherol, and tocotrienol

Masao Yamasaki, Eri Nishida, Shinsuke Nou, Hirofumi Tachibana, Koji Yamada

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12 Citations (Scopus)

Abstract

This study was performed to evaluate the isomer-specific cytotoxic effects of conjugated linoleic acid (CLA) on rat hepatoma dRLh-84 cells in vitro. A 10trans,12cis (10t,12c)-CLA showed a strong cytotoxic effect on dRLh-84 cells in culture, whereas no such effect was observed with 9cis,11trans (9c,11t)-CLA or linoleic acid. The optimum concentration for induction of cytotoxity by 10t,12c-CLA was 5 ti 10 μM, but the effect was alleviated at higher concentrations. Coincubation with oleic or palmitoleic acid and 10t,12c-CLA cancelled lhe cytotoxic effect, but other major saturated or polyunsaturated fully acids and eraidic acid did not interfere with 10t,12c-CLA-induced cytotoxity. The cytotoxic effect was also alleviated by α-tocopherol (α-toc) and α-tocotrienol but not by any other antioxidant regent examined. Significant cytotoxity of 10t,12c-CLA was detected after only a 15-min incubation, and the most noticeable effect was seen after 3 h. After incubation with 10t,12c-CLA at 10 μM, an additional 90 μM of 10t,12c-CLA or 100 μM of α-toc was also able to alleviate the cytotoxity. When cells were treated with 10 μM 10t,12c-CLA for more than 48 h, treatment with additional CLA or α-toc could not prevent cell death.

Original languageEnglish
Pages (from-to)239-244
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume41
Issue number7
DOIs
Publication statusPublished - Jul 1 2005
Externally publishedYes

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Tocotrienols
Conjugated Linoleic Acids
Tocopherols
Isomers
Rats
Hepatocellular Carcinoma
Fatty Acids
Modulation
Acids
Cell death
Linoleic Acid
Cell Death

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Cytotoxity of the trans10,cis12 isomer of conjugated linoleic acid on rat hepatoma and its modulation by other fatty acids, tocopherol, and tocotrienol. / Yamasaki, Masao; Nishida, Eri; Nou, Shinsuke; Tachibana, Hirofumi; Yamada, Koji.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 41, No. 7, 01.07.2005, p. 239-244.

Research output: Contribution to journalArticle

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abstract = "This study was performed to evaluate the isomer-specific cytotoxic effects of conjugated linoleic acid (CLA) on rat hepatoma dRLh-84 cells in vitro. A 10trans,12cis (10t,12c)-CLA showed a strong cytotoxic effect on dRLh-84 cells in culture, whereas no such effect was observed with 9cis,11trans (9c,11t)-CLA or linoleic acid. The optimum concentration for induction of cytotoxity by 10t,12c-CLA was 5 ti 10 μM, but the effect was alleviated at higher concentrations. Coincubation with oleic or palmitoleic acid and 10t,12c-CLA cancelled lhe cytotoxic effect, but other major saturated or polyunsaturated fully acids and eraidic acid did not interfere with 10t,12c-CLA-induced cytotoxity. The cytotoxic effect was also alleviated by α-tocopherol (α-toc) and α-tocotrienol but not by any other antioxidant regent examined. Significant cytotoxity of 10t,12c-CLA was detected after only a 15-min incubation, and the most noticeable effect was seen after 3 h. After incubation with 10t,12c-CLA at 10 μM, an additional 90 μM of 10t,12c-CLA or 100 μM of α-toc was also able to alleviate the cytotoxity. When cells were treated with 10 μM 10t,12c-CLA for more than 48 h, treatment with additional CLA or α-toc could not prevent cell death.",
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