D-RECS (1) a gene regulation system responding to protein kinase signal

Jun Oishi, Kenji Kawamura, Moeko Ijuin, Jeong Hun Kang, Daisuke Asai, Yuko Sato, Yoshiki Katayama

Research output: Contribution to conferencePaper

Abstract

We recently proposed the novel gene-regulation system responding to cAMP-dependent protein kinase (PKA). For this system, we used the cationic polymer which includes the oligopeptide that is the substrate of PKA. Since this polymer forms complexes with DNA, the gene expression was totally suppressed. However, when PKA is being continuously activated, anions were inserted in the polymer by phospholilating the peptides of side chains, the complex of DNA and polymer collapses and transcription of the gene is activated. We attempted to apply this system to living cell, and were successful to intracellular target signal-responsive regulation of delivered gene-expression.

Original languageEnglish
Number of pages1
Publication statusPublished - Oct 19 2006
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: May 24 2006May 26 2006

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period5/24/065/26/06

Fingerprint

Gene expression
Proteins
Polymers
DNA
Transcription
Peptides
Negative ions
Genes
Cells
Substrates

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Oishi, J., Kawamura, K., Ijuin, M., Kang, J. H., Asai, D., Sato, Y., & Katayama, Y. (2006). D-RECS (1) a gene regulation system responding to protein kinase signal. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.

D-RECS (1) a gene regulation system responding to protein kinase signal. / Oishi, Jun; Kawamura, Kenji; Ijuin, Moeko; Kang, Jeong Hun; Asai, Daisuke; Sato, Yuko; Katayama, Yoshiki.

2006. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.

Research output: Contribution to conferencePaper

Oishi, J, Kawamura, K, Ijuin, M, Kang, JH, Asai, D, Sato, Y & Katayama, Y 2006, 'D-RECS (1) a gene regulation system responding to protein kinase signal', Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan, 5/24/06 - 5/26/06.
Oishi J, Kawamura K, Ijuin M, Kang JH, Asai D, Sato Y et al. D-RECS (1) a gene regulation system responding to protein kinase signal. 2006. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.
Oishi, Jun ; Kawamura, Kenji ; Ijuin, Moeko ; Kang, Jeong Hun ; Asai, Daisuke ; Sato, Yuko ; Katayama, Yoshiki. / D-RECS (1) a gene regulation system responding to protein kinase signal. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.1 p.
@conference{b5ae3a067e574b2781355545a189b068,
title = "D-RECS (1) a gene regulation system responding to protein kinase signal",
abstract = "We recently proposed the novel gene-regulation system responding to cAMP-dependent protein kinase (PKA). For this system, we used the cationic polymer which includes the oligopeptide that is the substrate of PKA. Since this polymer forms complexes with DNA, the gene expression was totally suppressed. However, when PKA is being continuously activated, anions were inserted in the polymer by phospholilating the peptides of side chains, the complex of DNA and polymer collapses and transcription of the gene is activated. We attempted to apply this system to living cell, and were successful to intracellular target signal-responsive regulation of delivered gene-expression.",
author = "Jun Oishi and Kenji Kawamura and Moeko Ijuin and Kang, {Jeong Hun} and Daisuke Asai and Yuko Sato and Yoshiki Katayama",
year = "2006",
month = "10",
day = "19",
language = "English",
note = "55th SPSJ Annual Meeting ; Conference date: 24-05-2006 Through 26-05-2006",

}

TY - CONF

T1 - D-RECS (1) a gene regulation system responding to protein kinase signal

AU - Oishi, Jun

AU - Kawamura, Kenji

AU - Ijuin, Moeko

AU - Kang, Jeong Hun

AU - Asai, Daisuke

AU - Sato, Yuko

AU - Katayama, Yoshiki

PY - 2006/10/19

Y1 - 2006/10/19

N2 - We recently proposed the novel gene-regulation system responding to cAMP-dependent protein kinase (PKA). For this system, we used the cationic polymer which includes the oligopeptide that is the substrate of PKA. Since this polymer forms complexes with DNA, the gene expression was totally suppressed. However, when PKA is being continuously activated, anions were inserted in the polymer by phospholilating the peptides of side chains, the complex of DNA and polymer collapses and transcription of the gene is activated. We attempted to apply this system to living cell, and were successful to intracellular target signal-responsive regulation of delivered gene-expression.

AB - We recently proposed the novel gene-regulation system responding to cAMP-dependent protein kinase (PKA). For this system, we used the cationic polymer which includes the oligopeptide that is the substrate of PKA. Since this polymer forms complexes with DNA, the gene expression was totally suppressed. However, when PKA is being continuously activated, anions were inserted in the polymer by phospholilating the peptides of side chains, the complex of DNA and polymer collapses and transcription of the gene is activated. We attempted to apply this system to living cell, and were successful to intracellular target signal-responsive regulation of delivered gene-expression.

UR - http://www.scopus.com/inward/record.url?scp=33749844699&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749844699&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33749844699

ER -