We propose a novel method for gene therapy using drug or gene delivery system based on responses to intracellular signals, especially to the phosphorylation of protein kinase C (PKC) alpha. A cationic peptide that has high phosphoarylation sensitivity for PKC alpha was designed, and then a polymer with these peptides was synthesized. In the electrophoresis examination, the polymer containing the cationic peptide formed a stable complex with anionic DNA, but free DNA was found after phosphorylation by PKC alpha. In the intratumor delivery of polymer/DNA complex, the luciferase activity in the positive polymer for PKC alpha at charge ratios of 0.1 and 0.3 was 10- to 20-fold higher than that in the negative control polymer with a peptide which the phosphorylated site Serine was substituted by Alanine. These results indicate that the D-RECS is applicable to gene therapy in vivo.
|Number of pages||1|
|Publication status||Published - Oct 19 2006|
|Event||55th SPSJ Annual Meeting - Nagoya, Japan|
Duration: May 24 2006 → May 26 2006
|Other||55th SPSJ Annual Meeting|
|Period||5/24/06 → 5/26/06|
All Science Journal Classification (ASJC) codes