D-RECS (2) novel drug or gene delivery system based on responses to PKC alpha intracellular signals (D-RECS)

Jeong Hun Kang, Jun Oishi, Kenji Kawamura, Daisuke Asai, Riki Toita, Yuhua Jiang, Yuko Sato, Yoshiki Katayama

    Research output: Contribution to conferencePaperpeer-review

    Abstract

    We propose a novel method for gene therapy using drug or gene delivery system based on responses to intracellular signals, especially to the phosphorylation of protein kinase C (PKC) alpha. A cationic peptide that has high phosphoarylation sensitivity for PKC alpha was designed, and then a polymer with these peptides was synthesized. In the electrophoresis examination, the polymer containing the cationic peptide formed a stable complex with anionic DNA, but free DNA was found after phosphorylation by PKC alpha. In the intratumor delivery of polymer/DNA complex, the luciferase activity in the positive polymer for PKC alpha at charge ratios of 0.1 and 0.3 was 10- to 20-fold higher than that in the negative control polymer with a peptide which the phosphorylated site Serine was substituted by Alanine. These results indicate that the D-RECS is applicable to gene therapy in vivo.

    Original languageEnglish
    Number of pages1
    Publication statusPublished - Oct 19 2006
    Event55th SPSJ Annual Meeting - Nagoya, Japan
    Duration: May 24 2006May 26 2006

    Other

    Other55th SPSJ Annual Meeting
    Country/TerritoryJapan
    CityNagoya
    Period5/24/065/26/06

    All Science Journal Classification (ASJC) codes

    • Engineering(all)

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