D-RECS (2) novel drug or gene delivery system based on responses to PKC alpha intracellular signals (D-RECS)

Jeong Hun Kang, Jun Oishi, Kenji Kawamura, Daisuke Asai, Riki Toita, Yuhua Jiang, Yuko Sato, Yoshiki Katayama

Research output: Contribution to conferencePaper

Abstract

We propose a novel method for gene therapy using drug or gene delivery system based on responses to intracellular signals, especially to the phosphorylation of protein kinase C (PKC) alpha. A cationic peptide that has high phosphoarylation sensitivity for PKC alpha was designed, and then a polymer with these peptides was synthesized. In the electrophoresis examination, the polymer containing the cationic peptide formed a stable complex with anionic DNA, but free DNA was found after phosphorylation by PKC alpha. In the intratumor delivery of polymer/DNA complex, the luciferase activity in the positive polymer for PKC alpha at charge ratios of 0.1 and 0.3 was 10- to 20-fold higher than that in the negative control polymer with a peptide which the phosphorylated site Serine was substituted by Alanine. These results indicate that the D-RECS is applicable to gene therapy in vivo.

Original languageEnglish
Number of pages1
Publication statusPublished - Oct 19 2006
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: May 24 2006May 26 2006

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period5/24/065/26/06

Fingerprint

Genes
Peptides
Proteins
Polymers
Gene therapy
Phosphorylation
DNA
Electrophoresis

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Kang, J. H., Oishi, J., Kawamura, K., Asai, D., Toita, R., Jiang, Y., ... Katayama, Y. (2006). D-RECS (2) novel drug or gene delivery system based on responses to PKC alpha intracellular signals (D-RECS). Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.

D-RECS (2) novel drug or gene delivery system based on responses to PKC alpha intracellular signals (D-RECS). / Kang, Jeong Hun; Oishi, Jun; Kawamura, Kenji; Asai, Daisuke; Toita, Riki; Jiang, Yuhua; Sato, Yuko; Katayama, Yoshiki.

2006. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.

Research output: Contribution to conferencePaper

Kang, JH, Oishi, J, Kawamura, K, Asai, D, Toita, R, Jiang, Y, Sato, Y & Katayama, Y 2006, 'D-RECS (2) novel drug or gene delivery system based on responses to PKC alpha intracellular signals (D-RECS)' Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan, 5/24/06 - 5/26/06, .
Kang JH, Oishi J, Kawamura K, Asai D, Toita R, Jiang Y et al. D-RECS (2) novel drug or gene delivery system based on responses to PKC alpha intracellular signals (D-RECS). 2006. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.
Kang, Jeong Hun ; Oishi, Jun ; Kawamura, Kenji ; Asai, Daisuke ; Toita, Riki ; Jiang, Yuhua ; Sato, Yuko ; Katayama, Yoshiki. / D-RECS (2) novel drug or gene delivery system based on responses to PKC alpha intracellular signals (D-RECS). Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.1 p.
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AU - Kang, Jeong Hun

AU - Oishi, Jun

AU - Kawamura, Kenji

AU - Asai, Daisuke

AU - Toita, Riki

AU - Jiang, Yuhua

AU - Sato, Yuko

AU - Katayama, Yoshiki

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