D-RECS (3) development of functional polymers for inflammatory cells specific gene delivery system

Daisuke Asai, Yoko Shoji, Kenji Kawamura, Jun Oishi, Jeong Hun Kang, Yuko Sato, Takuro Niidome, Hideki Nakashima, Yoshiki Katayama

Research output: Contribution to conferencePaper

Abstract

Recently, we have developed and proposed a new system of the intracellular signal-responsive gene regulation as cell specific gene delivery, and termed the new concept D-RECS. In the present study, we focused IκB kinase that regulates NF-κB activation. According to D-RECS strategy, acrylamide polymers containing a substrate peptide for IκB kinase were designed and synthesized. The synthetic polymers were first examined in the gel retardation assay for IκB kinase β (IKKβ) using recombinant IKKβ. A polymer showed the efficient binding activity for plasmid DNA and significant responses to IKKβ. When the polymer-reporter plasmid DNA complex was transfected into living cells, and then the reporter activity was measured under the condition with or without TNFα stimulation, the gene expression with TNFα was stronger than that without TNFα stimulation. The differences in the reporter activity were found to be well-correlated to the TNFα stimulation.

Original languageEnglish
Number of pages1
Publication statusPublished - Oct 19 2006
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: May 24 2006May 26 2006

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period5/24/065/26/06

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All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Asai, D., Shoji, Y., Kawamura, K., Oishi, J., Kang, J. H., Sato, Y., ... Katayama, Y. (2006). D-RECS (3) development of functional polymers for inflammatory cells specific gene delivery system. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.