| Original language | English |
|---|---|
| Pages (from-to) | S78 |
| Journal | Developmental and Comparative Immunology |
| Volume | 18 |
| Issue number | SUPPL. 1 |
| DOIs | |
| Publication status | Published - 1994 |
All Science Journal Classification (ASJC) codes
- Immunology
- Developmental Biology
Access to Document
Other files and links
Cite this
- APA
- Standard
- Harvard
- Vancouver
- Author
- BIBTEX
- RIS
In: Developmental and Comparative Immunology, Vol. 18, No. SUPPL. 1, 1994, p. S78.
Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - D.4 Characterization of C3-derived fragments produced on activation of the carp complement
AU - Nakao, Miki
AU - Yano, Tomoki
N1 - Funding Information: Sylvia L. Smith ~, Barabara M. Webb', Mirta Riesgo', Steven D. Obenauf 2 'Medical Laboratory Sciences, Florida International University, Miami, FL 33199, USA, and 2Biological Sciences, Broward Community College, Davie, FL 33314, USA Recent studies have provided evidence for chemotactic factors and anaphylotoxins in zymosan-activated (10/zg/ml for 3 hr at 30°C) shark serum. Activated serum was fractionated by gel filtration and assayed for chemotactic and anaphylactic activity. Fractions were obtained which induced significant migratory response of human leucocytes in in vitro chemotaxis assays. One fraction (less than 17 kDa), in addition to chemotactic activity also induced muscle contraction of isolated rat ileum. This suggests the presence of peptides functionally similar to C5a and/or C3a. No activity was demonstrated in non-activated serum, The ability of shark serum to opsonize LPS-coated oil droplets was evaluated by measuring phagocytosis of droplets by human leucocytes. Ingestion and NBT reduction rates were determined spectrophotometrically and rates obtained were comparable to those of human serum. Shark serum was heated at varying temperatures for 15, 20 and 30 min; residual ACP (alternative pathway) and CCP (classical pathway) activity was measured in haemolytic assays using rabbit and sensitized sheep erythrocytes as targets respectively. Whereas all ACP activity was lost at 47°C in 20 min no significant loss of CCP activity occured at this temperature. Using heated serum (47°C, 20 min) as a reagent (RB), serum fractions, separated on Q-Sepharose FF, were identified which restored activity to RB by providing the missing heat labile factor. Supported by NIH grant #S06-GMOS205-09. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 1994
Y1 - 1994
UR - http://www.scopus.com/inward/record.url?scp=27244461567&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=27244461567&partnerID=8YFLogxK
U2 - 10.1016/0145-305X(94)90096-5
DO - 10.1016/0145-305X(94)90096-5
M3 - Article
AN - SCOPUS:27244461567
SN - 0145-305X
VL - 18
SP - S78
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
IS - SUPPL. 1
ER -