DAAM1 stabilizes epithelial junctions by restraining WAVE complex-dependent lateral membrane motility

Tamako Nishimura, Shoko Ito, Hiroko Saito, Sylvain Hiver, Kenta Shigetomi, Junichi Ikenouchi, Masatoshi Takeichi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Epithelial junctions comprise two subdomains, the apical junctional complex (AJC) and the adjacent lateral membrane contacts (LCs), that span the majority of the junction. The AJC is lined with circumferential actin cables, whereas the LCs are associated with less-organized actin filaments whose roles are elusive. We found that DAAM1, a formin family actin regulator, accumulated at the LCs, and its depletion caused dispersion of actin filaments at these sites while hardly affecting circumferential actin cables. DAAM1 loss enhanced the motility of LC-forming membranes, leading to their invasion of neighboring cell layers, as well as disruption of polarized epithelial layers. We found that components of the WAVE complex and its downstream targets were required for the elevation of LC motility caused by DAAM1 loss. These findings suggest that the LC membranes are motile by nature because of the WAVE complex, but DAAM1-mediated actin regulation normally restrains this motility, thereby stabilizing epithelial architecture, and that DAAM1 loss evokes invasive abilities of epithelial cells.

Original languageEnglish
Pages (from-to)559-573
Number of pages15
JournalJournal of Cell Biology
Volume215
Issue number4
DOIs
Publication statusPublished - Jan 1 2016

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Membranes
Actins
Actin Cytoskeleton
Epithelial Cells

All Science Journal Classification (ASJC) codes

  • Cell Biology

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DAAM1 stabilizes epithelial junctions by restraining WAVE complex-dependent lateral membrane motility. / Nishimura, Tamako; Ito, Shoko; Saito, Hiroko; Hiver, Sylvain; Shigetomi, Kenta; Ikenouchi, Junichi; Takeichi, Masatoshi.

In: Journal of Cell Biology, Vol. 215, No. 4, 01.01.2016, p. 559-573.

Research output: Contribution to journalArticle

Nishimura, Tamako ; Ito, Shoko ; Saito, Hiroko ; Hiver, Sylvain ; Shigetomi, Kenta ; Ikenouchi, Junichi ; Takeichi, Masatoshi. / DAAM1 stabilizes epithelial junctions by restraining WAVE complex-dependent lateral membrane motility. In: Journal of Cell Biology. 2016 ; Vol. 215, No. 4. pp. 559-573.
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