Daikenchuto ameliorates muscle hypercontractility in a murine T-Cell-mediated persistent gut motor dysfunction model

Hirotada Akiho, Kazuhiko Nakamura

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background and Aims: Low-grade inflammation and immunological alterations are evident in functional gastrointestinal disorders such as irritable bowel syndrome (IBS). We evaluated the effects of daikenchuto (DKT), a pharmaceutical grade Japanese herbal medicine, on the hypercontractility of intestinal smooth muscle persisting after acute inflammation induced by a T-cell-activating anti-CD3 antibody (αCD3). Methods: BALB/c mice were injected with αCD3 (12.5 μg, i.p.), and DKT (2.7 g/kg) was administered orally once daily for 1 week. The contraction of isolated small intestinal muscle strips and muscle cells was examined on day 7 after αCD3 injection. The gene and protein expressions in the small intestines were evaluated by real-time PCR and multiplex immunoassays, respectively, on days 1, 3 and 7 after αCD3 injection. Results: αCD3 injection resulted in significant increases in carbachol-evoked contractility in the muscle strips and isolated smooth muscle cells on day 7. DKT ameliorated the αCD3-induced muscle hypercontractility on day 7 in both the muscle strips and smooth muscle cells. αCD3 injection rapidly up- and downregulated the mRNA and protein expressions of pro- and anti-inflammatory cytokines, respectively. Although the influence of DKT on the mRNA expressions was moderate, the protein expressions of IL-13 and IL-17 were significantly decreased. Conclusions: We observed changes in the intestinal muscle contractility in muscle strips and muscle cells following resolution of inflammation in a T-cell-mediated model of enteropathy. The observed modulation of cytokine expression and function by DKT may lead to the development of new pharmacotherapeutic strategies aimed at a wide variety of gut motor dysfunction disorders.

Original languageEnglish
Pages (from-to)173-179
Number of pages7
JournalDigestion
Volume83
Issue number3
DOIs
Publication statusPublished - Jan 2011

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T-Lymphocytes
Muscles
Injections
Inflammation
Muscle Cells
Smooth Muscle Myocytes
Cytokines
Messenger RNA
Proteins
Interleukin-13
Interleukin-17
Herbal Medicine
Irritable Bowel Syndrome
Gastrointestinal Diseases
Carbachol
Immunoassay
Small Intestine
Smooth Muscle
dai-kenchu-to
Real-Time Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Gastroenterology

Cite this

Daikenchuto ameliorates muscle hypercontractility in a murine T-Cell-mediated persistent gut motor dysfunction model. / Akiho, Hirotada; Nakamura, Kazuhiko.

In: Digestion, Vol. 83, No. 3, 01.2011, p. 173-179.

Research output: Contribution to journalArticle

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