DAS-ELISA quantification of resting spores of Olpidium virulentus in roots and correlation between resting spore density in soil and severity of lettuce big-vein disease

Koji Nomiyama, Takahide Sasaya, Hiroyuki Sekiguchi, Keisuke Tomioka, Hideki Osaki, Minoru Takeshita, Toshiaki Takehara, Naruto Furuya, Kenichi Tsuchiya

Research output: Contribution to journalArticle

Abstract

A method to quantify resting spores of Olpidium virulentus, fungal vector of Mirafiori lettuce big-vein virus, which causes lettuce big-vein disease, in lettuce roots was developed using a DAS-ELISA with a polyclonal antibody against the resting spores and used to evaluate the relationship between the resting spore density in soil and disease severity. When lettuce seedlings were grown on soils containing a powder made from diseased roots with a known number of resting spores, symptoms developed from ca. 102 spores/g of soil or more. This simple method can be used in soil diagnosis and risk assessment of the disease.

Original languageEnglish
Pages (from-to)243-248
Number of pages6
JournalJournal of General Plant Pathology
Volume81
Issue number3
DOIs
Publication statusPublished - May 1 2015

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Olpidium
soil density
plant veins
lettuce
spores
enzyme-linked immunosorbent assay
Lettuce big-vein associated virus
soil
polyclonal antibodies
disease severity
signs and symptoms (plants)
risk assessment
powders
seedlings
methodology

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

DAS-ELISA quantification of resting spores of Olpidium virulentus in roots and correlation between resting spore density in soil and severity of lettuce big-vein disease. / Nomiyama, Koji; Sasaya, Takahide; Sekiguchi, Hiroyuki; Tomioka, Keisuke; Osaki, Hideki; Takeshita, Minoru; Takehara, Toshiaki; Furuya, Naruto; Tsuchiya, Kenichi.

In: Journal of General Plant Pathology, Vol. 81, No. 3, 01.05.2015, p. 243-248.

Research output: Contribution to journalArticle

Nomiyama, Koji ; Sasaya, Takahide ; Sekiguchi, Hiroyuki ; Tomioka, Keisuke ; Osaki, Hideki ; Takeshita, Minoru ; Takehara, Toshiaki ; Furuya, Naruto ; Tsuchiya, Kenichi. / DAS-ELISA quantification of resting spores of Olpidium virulentus in roots and correlation between resting spore density in soil and severity of lettuce big-vein disease. In: Journal of General Plant Pathology. 2015 ; Vol. 81, No. 3. pp. 243-248.
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AU - Osaki, Hideki

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