DAS-ELISA quantification of resting spores of Olpidium virulentus in roots and correlation between resting spore density in soil and severity of lettuce big-vein disease

Koji Nomiyama, Takahide Sasaya, Hiroyuki Sekiguchi, Keisuke Tomioka, Hideki Osaki, Minoru Takeshita, Toshiaki Takehara, Naruto Furuya, Kenichi Tsuchiya

Research output: Contribution to journalArticlepeer-review

Abstract

A method to quantify resting spores of Olpidium virulentus, fungal vector of Mirafiori lettuce big-vein virus, which causes lettuce big-vein disease, in lettuce roots was developed using a DAS-ELISA with a polyclonal antibody against the resting spores and used to evaluate the relationship between the resting spore density in soil and disease severity. When lettuce seedlings were grown on soils containing a powder made from diseased roots with a known number of resting spores, symptoms developed from ca. 102 spores/g of soil or more. This simple method can be used in soil diagnosis and risk assessment of the disease.

Original languageEnglish
Pages (from-to)243-248
Number of pages6
JournalJournal of General Plant Pathology
Volume81
Issue number3
DOIs
Publication statusPublished - May 1 2015

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

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