Data in support of protocol for rat single muscle-fiber isolation and culture

Yusuke Komiya, Judy E. Anderson, Mariko Akahoshi, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, Wataru Mizunoya

Research output: Contribution to journalArticle

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Abstract

This data article contains data related to the research article entitled, "Protocol for rat single muscle-fiber isolation and culture" by Komiya et al. [1]. It has yet to be shown whether adult myosin heavy chain (MyHC) isoforms are expressed at a readily detectable level in cultured myotubes. In this study, we examined whether adult MyHC isoforms could be detected in myotubes differentiated from rat satellite cells using a Western blotting assay and specific antibodies against slow MyHC, fast MyHC and pan-MyHC. Results showed that slow adult MyHC isoforms were faintly detected in myotubes, suggesting that rat myotubes express adult MyHC isoforms although that amount is very low.

Original languageEnglish
Pages (from-to)7-10
Number of pages4
JournalData in Brief
Volume4
DOIs
Publication statusPublished - Sep 1 2015

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Myosin Heavy Chains
Skeletal Muscle Fibers
Muscles
Protein Isoforms
Western Blotting
Antibodies
Research

All Science Journal Classification (ASJC) codes

  • General

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Data in support of protocol for rat single muscle-fiber isolation and culture. / Komiya, Yusuke; Anderson, Judy E.; Akahoshi, Mariko; Nakamura, Mako; Tatsumi, Ryuichi; Ikeuchi, Yoshihide; Mizunoya, Wataru.

In: Data in Brief, Vol. 4, 01.09.2015, p. 7-10.

Research output: Contribution to journalArticle

Komiya, Yusuke ; Anderson, Judy E. ; Akahoshi, Mariko ; Nakamura, Mako ; Tatsumi, Ryuichi ; Ikeuchi, Yoshihide ; Mizunoya, Wataru. / Data in support of protocol for rat single muscle-fiber isolation and culture. In: Data in Brief. 2015 ; Vol. 4. pp. 7-10.
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