Daughter Cell Identity Emerges from the Interplay of Cdc42, Septins, and Exocytosis

Satoshi Okada, Marcin Leda, Julia Hanna, Natasha S. Savage, Erfei Bi, Andrew B. Goryachev

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Asymmetric cell division plays a crucial role in cell differentiation, unequal replicative senescence, and stem cell maintenance. In budding yeast, the identities of mother and daughter cells begin to diverge at bud emergence when distinct plasma-membrane domains are formed and separated by a septin ring. However, the mechanisms underlying this transformation remain unknown. Here, we show that septins recruited to the site of polarization by Cdc42-GTP inhibit Cdc42 activity in a negative feedback loop, and this inhibition depends on Cdc42 GTPase-activating proteins. Combining live-cell imaging and computational modeling, we demonstrate that the septin ring is sculpted by polarized exocytosis, which creates a hole in the accumulating septin density and relieves the inhibition of Cdc42. The nascent ring generates a sharp boundary that confines the Cdc42 activity and exocytosis strictly to its enclosure and thus clearly delineates the daughter cell identity. Our findings define a fundamental mechanism underlying eukaryotic cell fate differentiation.

Original languageEnglish
Pages (from-to)148-161
Number of pages14
JournalDevelopmental Cell
Volume26
Issue number2
DOIs
Publication statusPublished - Jul 29 2013

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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