Decellularization of liver and organogenesis in rats

Nana Shirakigawa, Hiroyuki Ijima

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Recently, organ construction has been attempted using decellularized organs. In this study, we used decellularized rat liver to construct liver tissue by recellularization. The right lobe of the rat liver was decellularized with 4% Triton X-100 solution, recellularized with 10 7 rat hepatocytes, and albumin synthesis in the recellularized right lobe was observed. Therefore, we introduce a method of decellularizing rat liver, which retains its fine vascular structure after removal of all the cells, perform organogenesis using the decellularized liver, and evaluate the structural and functional properties of the products.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages271-281
Number of pages11
DOIs
Publication statusPublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1577
ISSN (Print)1064-3745

Fingerprint

Organogenesis
Liver
Octoxynol
Blood Vessels
Hepatocytes
Albumins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Shirakigawa, N., & Ijima, H. (2018). Decellularization of liver and organogenesis in rats. In Methods in Molecular Biology (pp. 271-281). (Methods in Molecular Biology; Vol. 1577). Humana Press Inc.. https://doi.org/10.1007/7651_2017_63

Decellularization of liver and organogenesis in rats. / Shirakigawa, Nana; Ijima, Hiroyuki.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 271-281 (Methods in Molecular Biology; Vol. 1577).

Research output: Chapter in Book/Report/Conference proceedingChapter

Shirakigawa, N & Ijima, H 2018, Decellularization of liver and organogenesis in rats. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1577, Humana Press Inc., pp. 271-281. https://doi.org/10.1007/7651_2017_63
Shirakigawa N, Ijima H. Decellularization of liver and organogenesis in rats. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 271-281. (Methods in Molecular Biology). https://doi.org/10.1007/7651_2017_63
Shirakigawa, Nana ; Ijima, Hiroyuki. / Decellularization of liver and organogenesis in rats. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 271-281 (Methods in Molecular Biology).
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