TY - CHAP
T1 - Decellularization of liver and organogenesis in rats
AU - Shirakigawa, Nana
AU - Ijima, Hiroyuki
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2017.
PY - 2018
Y1 - 2018
N2 - Recently, organ construction has been attempted using decellularized organs. In this study, we used decellularized rat liver to construct liver tissue by recellularization. The right lobe of the rat liver was decellularized with 4% Triton X-100 solution, recellularized with 107 rat hepatocytes, and albumin synthesis in the recellularized right lobe was observed. Therefore, we introduce a method of decellularizing rat liver, which retains its fine vascular structure after removal of all the cells, perform organogenesis using the decellularized liver, and evaluate the structural and functional properties of the products.
AB - Recently, organ construction has been attempted using decellularized organs. In this study, we used decellularized rat liver to construct liver tissue by recellularization. The right lobe of the rat liver was decellularized with 4% Triton X-100 solution, recellularized with 107 rat hepatocytes, and albumin synthesis in the recellularized right lobe was observed. Therefore, we introduce a method of decellularizing rat liver, which retains its fine vascular structure after removal of all the cells, perform organogenesis using the decellularized liver, and evaluate the structural and functional properties of the products.
UR - http://www.scopus.com/inward/record.url?scp=85057551264&partnerID=8YFLogxK
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U2 - 10.1007/7651_2017_63
DO - 10.1007/7651_2017_63
M3 - Chapter
C2 - 28808979
AN - SCOPUS:85057551264
T3 - Methods in Molecular Biology
SP - 271
EP - 281
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -