Decellularized liver as a practical scaffold with a vascular network template for liver tissue engineering

Nana Shirakigawa, Hiroyuki Ijima, Takayuki Takei

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

The construction of a functional liver-tissue equivalent using tissue engineering is a very important goal because the liver is a central organ in the body. However, the construction of functional organ-scale liver tissue is impossible because it requires a high-density blood vessel network. In this study, we focused on decellularization technology to solve this problem. Decellularized liver tissue with a fine vascular tree network template was obtained using Triton X-100. The distance between each vascular structure was less than 1 mm. Endothelialization of the blood vessel network with human umbilical vein endothelial cells (HUVECs) was successfully performed without any leakage of HUVECs to the outside of the vessel structure. Furthermore, hepatocytes/spheroids could be located around the blood vessel structure. This study indicates that decellularized liver tissue is a potential scaffold for creating a practical liver tissue using tissue engineering technology.

Original languageEnglish
Pages (from-to)546-551
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume114
Issue number5
DOIs
Publication statusPublished - Nov 2012

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint

Dive into the research topics of 'Decellularized liver as a practical scaffold with a vascular network template for liver tissue engineering'. Together they form a unique fingerprint.

Cite this