Tissue Engineering consists of cells, a scaffold and cytokines. Decellularization represents the removal of cells from tissues or organs. Recently, decellularized tissue has been investigated as a scaffold for tissue engineering, termed decellularized tissue engineering. Importantly, the decellularized organ retains its original structure, which is then used as a template for organ construction. The decellularized organ also retains the tissue-specific extracellular matrix. Therefore, decellularized tissue can be used as a matrix to provide a suitable microenvironment for inoculated cells. Based on these concepts, the reconstruction of tissues/organs with decellularized tissue/organ has been attempted using decellularized tissue engineering. In this chapter, we introduce the typical methods used, history and attainment level for the reconstruction of specific tissues/organs. First, the different decellularized techniques and characteristics are introduced. Then, the commonly used analysis methods and cautionary points during decellularization and reconstruction with decellularized tissues/organs are explained. Next, the specific methods and characteristics of decellularized tissue engineering for specific tissues/organs are introduced. In these sections, the current conditions, problems and future work are explained. Finally, we conclude with a summary of this chapter.