Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae

Masataka Hirasaki, Fumika Nakamura, Kazuo Yamagishi, Minori Numamoto, Yukiko Shimada, Keigo Uehashi, Shigeru Muta, Minetaka Sugiyama, Yoshinobu Kaneko, Satoru Kuhara, Satoshi Harashima

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6 Citations (Scopus)

Abstract

Expression profiles of protein phosphatase (PPase) disruptants were analyzed by use of Pearson's correlation coefficient to find profiles that correlated with those of 316 Reference Gene (RG) disruptants harboring deletions in genes with known functions. Twenty-six Δppase disruptants exhibited either a positive or negative correlation with 94 RG disruptants when the p value for Pearson's correlation coefficient was > 0.2. Some of the predictions that arose from this analysis were tested experimentally and several new Δppase phenotypes were found. Notably, Δsit4 and Δsiw14 disruptants exhibited hygromycin B sensitivity, Δsit4 and Δptc1 disruptants grew slowly on glycerol medium, the Δptc1 disruptant was found to be sensitive to calcofluor white and congo red, while the Δppg1 disruptant was found to be sensitive to congo red. Because on-going analysis of expression profiles of Saccharomyces cerevisiae disruptants is rapidly generating new data, we suggest that the approach used in the present study to explore PPase function is also applicable to other genes.

Original languageEnglish
Pages (from-to)433-441
Number of pages9
JournalJournal of Bioscience and Bioengineering
Volume109
Issue number5
DOIs
Publication statusPublished - May 2010

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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    Hirasaki, M., Nakamura, F., Yamagishi, K., Numamoto, M., Shimada, Y., Uehashi, K., Muta, S., Sugiyama, M., Kaneko, Y., Kuhara, S., & Harashima, S. (2010). Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae. Journal of Bioscience and Bioengineering, 109(5), 433-441. https://doi.org/10.1016/j.jbiosc.2009.10.023