TY - JOUR
T1 - Decolorization of azo and anthraquinone dyes in hydrophobic organic media using microperoxidase-11 entrapped in reversed micelles
AU - Okazaki, Shin Ya
AU - Nagasawa, Shin Ichiro
AU - Goto, Masahiro
AU - Furusaki, Shintaro
AU - Wariishi, Hiroyuki
AU - Tanaka, Hiroo
N1 - Funding Information:
This research was supported by the Regional Science Promoter Program from the FUKUOKA Industry, Science and Technology Foundation (to HW), a Grant-in-Aid for Scientific Research (B; 10555284) from the Japan Society for the Promotion of Science (JSPS) (to MG and SF), and the Proposal-Based New Industry Creative Type Technology R&D Promotion Program from the New Energy and Industrial Technology Development Organization (NEDO) of Japan (to MG, SF, HW, and HT). SO was supported by Research Fellowships of JSPS for Young Scientists.
PY - 2002/12
Y1 - 2002/12
N2 - Microperoxidase-11 (MP-11), which is a heme-containing undecapeptide derived from horse heart cytochrome c, was utilized as a peroxidative catalyst in the system of bis(2-ethylhexyl)sulphosuccinate sodium salt (AOT)-reversed micelles to oxidatively decolorize water-insoluble dyes in isooctane. MP-11 entrapped in the reversed micelles exhibited a peroxidative activity in the presence of H2O2, effectively catalyzing the oxidative decolorization of an azo dye Solvent Yellow 7 and an anthraquinone dye Solvent Blue 11 in the hydrophobic organic solvent. H2O2-derived heme bleaching was successfully suppressed by replacing H2O2 with an organic hydroperoxide, tert-butyl hydroperoxide (t-BuOOH), thus sustaining a stable decolorization activity in the system. To optimize the reversed micellar system, the effect of the pH and the molar ratio of H2O/AOT hydration degree (Wo) was examined, indicating that MP-11 exhibited a maximal decolorization activity at pH 8-10 with the Wo value of 20.
AB - Microperoxidase-11 (MP-11), which is a heme-containing undecapeptide derived from horse heart cytochrome c, was utilized as a peroxidative catalyst in the system of bis(2-ethylhexyl)sulphosuccinate sodium salt (AOT)-reversed micelles to oxidatively decolorize water-insoluble dyes in isooctane. MP-11 entrapped in the reversed micelles exhibited a peroxidative activity in the presence of H2O2, effectively catalyzing the oxidative decolorization of an azo dye Solvent Yellow 7 and an anthraquinone dye Solvent Blue 11 in the hydrophobic organic solvent. H2O2-derived heme bleaching was successfully suppressed by replacing H2O2 with an organic hydroperoxide, tert-butyl hydroperoxide (t-BuOOH), thus sustaining a stable decolorization activity in the system. To optimize the reversed micellar system, the effect of the pH and the molar ratio of H2O/AOT hydration degree (Wo) was examined, indicating that MP-11 exhibited a maximal decolorization activity at pH 8-10 with the Wo value of 20.
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U2 - 10.1016/S1369-703X(02)00074-8
DO - 10.1016/S1369-703X(02)00074-8
M3 - Article
AN - SCOPUS:0036883474
VL - 12
SP - 237
EP - 241
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
SN - 1369-703X
IS - 3
ER -