Decrease in circulating Th17 cells correlates with increased levels of CCL17, IgE and eosinophils in atopic dermatitis

Sayaka Hayashida, Hiroshi Uchi, Yoichi Moroi, Masutaka Furue

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Background: Clinical significance of circulating CD4+ T cell subsets, including T-helper (Th)1, Th2, Th17 and regulatory T (Treg) cells, in patients with atopic dermatitis (AD) remains unclear. No previous studies have simultaneously evaluated the four T cell subset profiles in AD. Objective: The aim of the present study was to explore whether the percentage of these four subsets of CD4+ T cells correlate to the severity parameters of AD patients. Methods: Intracellular expression of interferon (IFN)-γ, interleukin (IL)-4, IL-17 and forkhead box P3 (Foxp3) in CD4+ T cells was evaluated in peripheral blood mononuclear cells from normal controls and patient with AD as well as with chronic eczema using a flow cytometer. Serum CCL17 levels were measured as an objective severity parameter of AD together with percentage of eosinophils and serum IgE levels. Results: In AD patients, the number of Th1 (IFN-γ+) and Th17 (IL-17+) subsets was significantly decreased, but that of Th2 (IL-4+) and Treg (Foxp3+) subsets was similar to that of normal controls. The T cell subset profiles of patients with chronic eczema were not different with those of normal controls. The frequency of Th17cells, particularly that of the IFN-γnegaIL-17+ subset, showed a significant negative correlation with CCL17, IgE and eosinophil levels in AD patients. This was, however, not the case in Th1, Th2 and Treg cells. Conclusion: Decreased circulating Th17 cells might contribute to activity of AD.

Original languageEnglish
Pages (from-to)180-186
Number of pages7
JournalJournal of Dermatological Science
Volume61
Issue number3
DOIs
Publication statusPublished - Mar 1 2011

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Th17 Cells
Atopic Dermatitis
T-cells
Eosinophils
Immunoglobulin E
T-Lymphocyte Subsets
Interferons
Interleukin-17
Eczema
Regulatory T-Lymphocytes
Interleukin-4
Serum
Blood Cells
Blood
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

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Decrease in circulating Th17 cells correlates with increased levels of CCL17, IgE and eosinophils in atopic dermatitis. / Hayashida, Sayaka; Uchi, Hiroshi; Moroi, Yoichi; Furue, Masutaka.

In: Journal of Dermatological Science, Vol. 61, No. 3, 01.03.2011, p. 180-186.

Research output: Contribution to journalArticle

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N2 - Background: Clinical significance of circulating CD4+ T cell subsets, including T-helper (Th)1, Th2, Th17 and regulatory T (Treg) cells, in patients with atopic dermatitis (AD) remains unclear. No previous studies have simultaneously evaluated the four T cell subset profiles in AD. Objective: The aim of the present study was to explore whether the percentage of these four subsets of CD4+ T cells correlate to the severity parameters of AD patients. Methods: Intracellular expression of interferon (IFN)-γ, interleukin (IL)-4, IL-17 and forkhead box P3 (Foxp3) in CD4+ T cells was evaluated in peripheral blood mononuclear cells from normal controls and patient with AD as well as with chronic eczema using a flow cytometer. Serum CCL17 levels were measured as an objective severity parameter of AD together with percentage of eosinophils and serum IgE levels. Results: In AD patients, the number of Th1 (IFN-γ+) and Th17 (IL-17+) subsets was significantly decreased, but that of Th2 (IL-4+) and Treg (Foxp3+) subsets was similar to that of normal controls. The T cell subset profiles of patients with chronic eczema were not different with those of normal controls. The frequency of Th17cells, particularly that of the IFN-γnegaIL-17+ subset, showed a significant negative correlation with CCL17, IgE and eosinophil levels in AD patients. This was, however, not the case in Th1, Th2 and Treg cells. Conclusion: Decreased circulating Th17 cells might contribute to activity of AD.

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