Decrease of interleukin-10-producing T cells in the peripheral blood of severe unstable atopic asthmatics

Koichiro Matsumoto, Hiromasa Inoue, Satoru Fukuyama, Miyuki Tsuda, Tomomi Ikegami, Atsuko Kibe, Yuki Yoshiura, Masashi Komori, Naotaka Hamasaki, Hisamichi Aizawa, Yoichi Nakanishi

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Background: Although IL-10 is known as an immunoregulatory cytokine produced by various cells including T cells, its basic profile in atopic asthma remains uncertain. Objective: The profiles of IL-10 production in circulating CD4+ T cells of atopic asthmatics were investigated with respect to clinical severity. Methods: Forty atopic asthmatics were divided into three groups: mild, and severe but stable and severe unstable asthmatics. Eosinophils were counted in the peripheral blood and sputum, and exhaled nitric oxide was assessed. PBMCs were stimulated with or without anti-CD3 and anti-CD28 antibodies and then processed for detecting IL-10-producing CD4+ cells using flow cytometry. Results: There was no difference in the eosinophil count in blood or sputum and in nitric oxide level among the three groups. IL-10-producing CD4+ cells were mainly detected in a CD45RO+ memory population. The frequency of IL-10-producing cells after stimulation was significantly lower in the severe unstable group compared to the mild group. In addition, the frequency of IL-10-producing cells in the severe unstable group was significantly lower than that in the severe stable group despite the fact that both groups received similar treatments with high-dose inhaled corticosteroids. The IL-10 production of CD4+CD45RO+ cells in response to dexamethasone did not differ among the three groups. Conclusions: IL-10-producing CD4+CD45RO+ cells in the peripheral blood are decreased in severe unstable asthmatics, which is not explained by the effect of high-dose inhaled corticosteroid medication.

Original languageEnglish
Pages (from-to)295-302
Number of pages8
JournalInternational Archives of Allergy and Immunology
Volume134
Issue number4
DOIs
Publication statusPublished - 2004

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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