Decrease of nuclear protein phosphatase 1 activity and induction of mitotic arrest and apoptosis by a marine microalgal polysaccharide in human myeloid leukemia U937 cells

Kenichi Sogawa, Takahisa Yamada, Youji Muramatsu, Tomoki Sumida, Hiroyuki Hamakawa, Hironobu Oda, Hidenori Miyake, Seiki Tashiro, Masahiro Matsuda, Kozo Matsumoto, Koichi Okutani

Research output: Contribution to journalArticle

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Abstract

An extracellular polysaccharide, which we designate GA3P, produced from a marine microalga dinoflagellate Gymnodinium sp. A3, has been previously reported to induce apoptosis in lymphoid and myeloid cell lines. We found that the GA3P accumulates cells into the mitotic phase of the cell cycle and decreases nuclear protein phosphatase 1 (PP1) activity in a dose-dependent manner in myeloid leukemia U937 cells. Dose-dependent patterns in the decrease of nuclear PP1 activity and in the accumulation of cells into mitotic phase or apoptotic status by the GA3P were concordant with each other, indicating that the decrease of nuclear PP1 activity at least mediates some of the etiological steps in development of mitotic arrest and apoptosis induced by the GA3P. In addition, the GA3P repressed the expression of protein levels of the PP1 catalytic subunit isoform PP1γ1. We thus suggest that the decrease of nuclear PP1 activity is due to down-regulation of the protein levels of the PP 1γ1.

Original languageEnglish
Pages (from-to)267-282
Number of pages16
JournalResearch Communications in Molecular Pathology and Pharmacology
Volume99
Issue number3
Publication statusPublished - Jan 1 1998
Externally publishedYes

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Protein Phosphatase 1
U937 Cells
Myeloid Leukemia
Phosphoprotein Phosphatases
Polysaccharides
Apoptosis
Dinoflagellida
Myeloid Cells
Cell Cycle
Protein Isoforms
Proteins
Down-Regulation
Lymphocytes
Cell Line

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology

Cite this

Decrease of nuclear protein phosphatase 1 activity and induction of mitotic arrest and apoptosis by a marine microalgal polysaccharide in human myeloid leukemia U937 cells. / Sogawa, Kenichi; Yamada, Takahisa; Muramatsu, Youji; Sumida, Tomoki; Hamakawa, Hiroyuki; Oda, Hironobu; Miyake, Hidenori; Tashiro, Seiki; Matsuda, Masahiro; Matsumoto, Kozo; Okutani, Koichi.

In: Research Communications in Molecular Pathology and Pharmacology, Vol. 99, No. 3, 01.01.1998, p. 267-282.

Research output: Contribution to journalArticle

Sogawa, Kenichi ; Yamada, Takahisa ; Muramatsu, Youji ; Sumida, Tomoki ; Hamakawa, Hiroyuki ; Oda, Hironobu ; Miyake, Hidenori ; Tashiro, Seiki ; Matsuda, Masahiro ; Matsumoto, Kozo ; Okutani, Koichi. / Decrease of nuclear protein phosphatase 1 activity and induction of mitotic arrest and apoptosis by a marine microalgal polysaccharide in human myeloid leukemia U937 cells. In: Research Communications in Molecular Pathology and Pharmacology. 1998 ; Vol. 99, No. 3. pp. 267-282.
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AU - Hamakawa, Hiroyuki

AU - Oda, Hironobu

AU - Miyake, Hidenori

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AU - Okutani, Koichi

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